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Priming of insulin ...
Priming of insulin granules for exocytosis by granular Cl(-) uptake and acidification
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- Barg, Sebastian (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Physiological Sciences, Lund University, Lund
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- Huang, Ping (författare)
- University of Chicago, Department of Neurobiology, Pharmacology and Physiology, Chicago
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- Eliasson, Lena (författare)
- Lund University,Lunds universitet,Diabetes - öcellsexocytos,Forskargrupper vid Lunds universitet,Diabetes - Islet Cell Exocytosis,Lund University Research Groups,Department of Physiological Sciences, Lund University, Lund
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- Nelson, Deborah J (författare)
- University of Chicago, Department of Neurobiology, Pharmacology and Physiology, Chicago
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- Obermüller, Stefanie (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Physiological Sciences, Lund University, Lund
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- Rorsman, Patrik (författare)
- Lund University,Lunds universitet,Islet cell physiology,Forskargrupper vid Lunds universitet,Lund University Research Groups,Department of Physiological Sciences, Lund University, Lund
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- Thévenod, Frank (författare)
- Physiologisches Institut, Universität des Saarlandes, Homburg/Saar
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- Renström, Erik (författare)
- Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups,Department of Physiological Sciences, Lund University, Lund
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: Journal of Cell Science. - 0021-9533 .- 1477-9137. ; 114:Pt 11, s. 2145-54
- Relaterad länk:
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http://jcs.biologist...
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https://urn.kb.se/re...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- ATP-dependent priming of the secretory granules precedes Ca(2+)-regulated neuroendocrine secretion, but the exact nature of this reaction is not fully established in all secretory cell types. We have further investigated this reaction in the insulin-secreting pancreatic B-cell and demonstrate that granular acidification driven by a V-type H(+)-ATPase in the granular membrane is a decisive step in priming. This requires simultaneous Cl(-) uptake through granular ClC-3 Cl(-) channels. Accordingly, granule acidification and priming are inhibited by agents that prevent transgranular Cl(-) fluxes, such as 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and an antibody against the ClC-3 channels, but accelerated by increases in the intracellular ATP:ADP ratio or addition of hypoglycemic sulfonylureas. We suggest that this might represent an important mechanism for metabolic regulation of Ca(2+)-dependent exocytosis that is also likely to be operational in other secretory cell types.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- ClC-3 channels
- Exocytosis
- Granular pH
- Insulin
- Sulfonylureas
- ClC-3 channels
- Exocytosis
- Sulfonylureas
- Insulin
- Granular pH
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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