Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections

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Lodén, Henrik,1972-Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi (författare)

Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections

BokEngelska2008

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Uppsala :Acta Universitatis Upsaliensis,2008
60 s.
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:uu-8841
ISBN:9789155472115
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8841URI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:vet swepub-contenttype
Ämneskategori:dok swepub-publicationtype

Serie

Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy,1651-6192 ;75

Anmärkningar

Different multiple-injection methodologies and the partial filling technique (PFT) have been utilized for separation of pharmaceuticals by capillary elec-trophoresis. In multiple-injection capillary zone electrophoresis (MICZE), the samples and all standards, used for construction of the calibration curve, are analyzed within a single run. Four different modes of MICZE have been described by means of equations, which were experimentally verified. The developed equations facilitate the transfer from conventional single-injection CZE to one or more of these MICZE-modes, depending on the selectivity between the analyte and the injection marker. The applicability of two of these modes was then demonstrated by quantification of buserelin and salbutamol, re-spectively in commercially available pharmaceutical products. The content of buserelin in an injection solution was determined to 0.94 mg/ml, which only deviated slightly from the declared concentration (1 mg/ml). An alter-native mode of MICZE, offering a higher number of sequential sample injec-tions, was then utilized for single-run determination of salbutamol in 15 tab-lets, with a labelled content of 8 mg. The average content of the tablets was determined to 7.8 mg, with an intra-tablet variation of 3 % or less. Moreover, UV- and mass-spectrometric detection of enantiomeric amines, resolved by non-aqueous capillary electrophoresis (NACE), was demon-strated. Separation of enantiomeric amines was achieved using the chiral selector (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid, (-)-DIKGA. Introduction of the non-volatile (-)-DIKGA into the mass-spectrometer was avoided by using the PFT, where the capillary is only partially filled with electrolyte containing the chiral selector.

Ämnesord och genrebeteckningar

Capillary Coating
Chiral Separation
Enantiomers
Mass-spectrometric Detection
Method Validation
Multiple-injection Capillary Zone Electrophoresis
Non-aqueous Capillary Electrophoresis
Organic Solvents
Pharmaceutical Analysis
Quantitative Analysis

Biuppslag (personer, institutioner, konferenser, titlar ...)

Pettersson, Curt (preses)
Arvidsson, Torbjörn (preses)
Amini, Ahmad (preses)
Stefansson, Morgan,DocentAstraZeneca R&D, Mölndal (opponent)
Uppsala universitetAvdelningen för analytisk farmaceutisk kemi (creator_code:org_t)

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Av författaren/redakt...: Lodén, Henrik, 1 ...; Pettersson, Curt; Arvidsson, Torbj ...; Amini, Ahmad; Stefansson, Morg ...

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Av lärosätet: Uppsala universitet

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