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Requirement of the ...
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Lindholm, Cecilia KUppsala universitet,Institutionen för medicinsk cellbiologi
(författare)
Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the Interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
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Elsevier BV,1999
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:uu-89705
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-89705URI
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https://doi.org/10.1074/jbc.274.39.28050DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Stimulation of the T cell antigen receptor (TCR) induces tyrosine phosphorylation of numerous intracellular proteins. We have recently investigated the role of the adaptor protein Shb in the early events of T cell signaling and observed that Shb associates with Grb2, linker for activation of T cells (LAT) and the TCR zeta-chain in Jurkat cells. We now report that Shb also associates with phospholipase C-gamma1 (PLC-gamma1) in these cells. Overexpression of Src homology 2 domain defective Shb caused diminished phosphorylation of LAT and consequently the activation of mitogen-activated protein kinases was decreased upon TCR stimulation. In addition, the Shb mutant also blocked phosphorylation of PLC-gamma1 and the increase in cytoplasmic Ca(2+) following TCR stimulation. Nuclear factor for activation of T cells is a major target for Ras and calcium signaling pathways in T cells following TCR stimulation, and the overexpression of the mutant Shb prevented TCR-dependent activation of the nuclear factor for activation of T cells. Consequently, endogenous interleukin-2 production was decreased under these conditions. The results indicate a role for Shb as a link between the TCR and downstream signaling events involving LAT and PLC-gamma1 and resulting in the activation of transcription of the interleukin-2 gene.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Gylfe, Erik,1947-Uppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)erikgylf
(författare)
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Zhang, Weigu
(författare)
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Samelson, Lawrence E
(författare)
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Welsh, MichaelUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)michwels
(författare)
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Uppsala universitetInstitutionen för medicinsk cellbiologi
(creator_code:org_t)
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Ingår i:Journal of Biological Chemistry: Elsevier BV274:39, s. 28050-280570021-92581083-351X
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