Immunofluorescence localization of nuclear retinoid receptors in psoriasis versus normal human skin

Psoriasis responds favourably to treatment with retinoids but the cellular pathways mediating these effects are poorly understood. Retinoids regulate keratinocyte proliferation and maturation via binding to nuclear retinoic acid receptors (mainly RARalpha and RARgamma) which form heterodimers with the 9-cis-RA receptor, RXRalpha. We have previously shown that mRNA expression of RARalpha and RXRalpha is down-regulated in psoriatic lesions as compared with non-lesional human skin. In the present study, we investigated the protein expression of RARalpha, RARgamma and RXRalpha in normal and psoriatic skin using indirect immunofluorescence analysis. Epidermal keratinocytes of normal and non-lesional psoriatic skin displayed similar nuclear localization of all three receptors; RARalpha was detected with decreasing intensity from basal to suprabasal layers, RARgamma showed the opposite trend, whereas RXRalpha was evenly expressed throughout the epidermis. In lesional psoriatic skin, however, all three receptor proteins showed a much higher staining intensity in the lower half of the epidermis; in particular, RARalpha immunoreactivity was low or even absent in the upper layers of epidermis. The results support the idea that psoriasis is associated with abnormal retinoid signalling in lesional epidermis.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
Adult
Aged
Aged; 80 and over
Female
Fluorescent Antibody Technique
Humans
Keratinocytes
Male
Middle Aged
Psoriasis/*immunology
Receptors; Retinoic Acid/immunology
Research Support; Non-U.S. Gov't
Retinoid X Receptor alpha/immunology
Retinoid X Receptors/*immunology
Dermatology and venerology
Dermatologi och venerologi
Dermatology and Venerology
Dermatologi och venereologi

Rollman, OlaUppsala universitet,Dermatologi och venereologi (författare)
Vahlquist, AndersUppsala universitet,Dermatologi och venereologi (författare)
Törmä, HansUppsala universitet,Dermatologi och venereologi(Swepub:uu)hanstorm (författare)
Uppsala universitetDermatologi och venereologi (creator_code:org_t)

Ingår i:Acta Dermato-Venereologica: Medical Journals Sweden AB84:5, s. 363-3690001-55551651-2057

Av författaren/redakt...: Karlsson, Teresa; Rollman, Ola; Vahlquist, Ander ...; Törmä, Hans

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Dermatologi och ...

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