Improved lectin-mediated immobilization of human red blood cells in superporous agarose beads

Gottschalk, IngoUppsala universitet,Institutionen för naturvetenskaplig biokemi (author)

A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin–agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Wheat germ agglutinin
Cytochalasin B
GLUT1
Immobilization
Superporous agarose
Wheat germ agglutinin
Cytochalasin B
GLUT1

Gustavsson, Per-ErikLund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)tbk-peg (author)
Ersson, BoUppsala universitet,Institutionen för naturvetenskaplig biokemi (author)
Lundahl, PerUppsala universitet,Institutionen för ytbioteknik med Centrum för ytbioteknik (author)
Uppsala universitetInstitutionen för naturvetenskaplig biokemi (creator_code:org_t)

By the author/editor: Gottschalk, Ingo; Gustavsson, Per- ...; Ersson, Bo; Lundahl, Per

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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