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  • Feierberg, IsabellaUppsala universitet,Institutionen för cell- och molekylärbiologi (author)

Energetics of the proposed rate-determining step of the glyoxalase I reaction

  • Article/chapterEnglish1999

Publisher, publication year, extent ...

  • 1999
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-90163
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-90163URI
  • https://doi.org/10.1016/S0014-5793(99)00703-6DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • The proposed rate-limiting step of the reaction catalyzed by glyoxalase I is the proton abstraction from the C1 carbon atom of the substrate by a glutamate residue, resulting in a high-energy enolate intermediate. This proton transfer reaction was modelled using molecular dynamics and free energy perturbation simulations, with the empirical valence bond method describing the potential energy surface of the system. The calculated rate constant for the reaction is approximately 300-1500 s(-1) with Zn2+, Mg2+ or Ca2+ bound to the active site, which agrees well with observed kinetics of the enzyme. Furthermore, the results imply that the origin of the catalytic rate enhancement is mainly associated with enolate stabilization by the metal ion.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Cameron, Alexander D (author)
  • Åqvist, JohanUppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)johanaq (author)
  • Uppsala universitetInstitutionen för cell- och molekylärbiologi (creator_code:org_t)

Related titles

  • In:FEBS Letters453:1-2, s. 90-940014-57931873-3468

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