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Phospholipase C act...
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Krjukova, JelenaUppsala universitet,Fysiologi
(författare)
Phospholipase C activator m-3M3FBS affects Ca2+ homeostasis independently of phospholipase C activation
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
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2009-02-17
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Wiley,2004
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-91904
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-91904URI
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https://doi.org/10.1038/sj.bjp.0705911DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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In this study, we have investigated responses to the phospholipase C (PLC) activator m-3M3FBS in SH-SY5Y human neuroblastoma cells. As measured using fura-2, m-3M3FBS caused a slowly developing - full response was obtained within 4-6 min - Ca(2+) elevation both in the presence and absence of extracellular Ca(2+), indicating Ca(2+) release from intracellular stores, putatively from endoplasmic reticulum and mitochondria. PLC activity was also measured using two methods, the classical ion-exchange separation and the more novel fluorescent real-time method. In the time frame in which m-3M3FBS caused Ca(2+) elevation (up to 7 min), no PLC activation was detected. Instead, more than 20 min were required to see any inositol phosphate generation in response to m-3M3FBS. m-3M3FBS also interfered with store-operated Ca(2+) influx and Ca(2+) extrusion. In conclusion, m-3M3FBS cannot be considered either potent or specific PLC activator.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Holmqvist, TomasUppsala universitet,Fysiologi
(författare)
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Danis, AlexanderUppsala universitet,Fysiologi
(författare)
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Åkerman, KarilUppsala universitet,Fysiologi
(författare)
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Kukkonen, JyrkiUppsala universitet,Fysiologi
(författare)
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Uppsala universitetFysiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:British Journal of Pharmacology: Wiley143:1, s. 3-70007-11881476-5381
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