Search: id:"swepub:oai:DiVA.org:uu-92987" >
Mitochondrial seque...
-
Andréasson, HannaUppsala universitet,Institutionen för immunologi, genetik och patologi
(author)
Mitochondrial sequence analysis for forensic identification using Pyrosequencing technology
- Article/chapterEnglish2002
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-92987
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-92987URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Over recent years, requests for mtDNA analysis in the field of forensic medicine have notably increased, and the results of such analyses have proved to be very useful in forensic cases where nuclear DNA analysis cannot be performed. Traditionally, mtDNA has been analyzed by DNA sequencing of the two hypervariable regions, HVI and HVII, in the D-loop. DNA sequence analysis using the conventional Sanger sequencing is very robust but time consuming and labor intensive. By contrast, mtDNA analysis based on the pyrosequencing technology provides fast and accurate results from the human mtDNA present in many types of evidence materials in forensic casework. The assay has been developed to determine polymorphic sites in the mitochondrial D-loop as well as the coding region to further increase the discrimination power of mtDNA analysis. The pyrosequencing technology for analysis of mtDNA polymorphisms has been tested with regard to sensitivity, reproducibility, and success rate when applied to control samples and actual casework materials. The results show that the method is very accurate and sensitive; the results are easily interpreted and provide a high success rate on casework samples. The panel of pyrosequencing reactions for the mtDNA polymorphisms were chosen to result in an optimal discrimination power in relation to the number of bases determined.
Added entries (persons, corporate bodies, meetings, titles ...)
-
Asp, AllanUppsala universitet,Tillämpad kärnfysik
(author)
-
Alderborn, AndersUppsala universitet,Medicinska och farmaceutiska vetenskapsområdet
(author)
-
Gyllensten, UlfUppsala universitet,Genomik
(author)
-
Allen, MarieUppsala universitet,Genomik(Swepub:uu)marialle
(author)
-
Uppsala universitetInstitutionen för immunologi, genetik och patologi
(creator_code:org_t)
Related titles
-
In:BioTechniques32:1, s. 124-6, 128, 130-30736-62051940-9818
Internet link
Find in a library
To the university's database