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  • Ericsson, OlleUppsala universitet,Institutionen för genetik och patologi (author)

A dual-tag microarray platform for high-performance nucleic acid and protein analyses

  • Article/chapterEnglish2008

Publisher, publication year, extent ...

  • 2008-03-16
  • Oxford University Press (OUP),2008
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-96777
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-96777URI
  • https://doi.org/10.1093/nar/gkn106DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • DNA microarrays serve to monitor a wide range of molecular events, but emerging applications like measurements of weakly expressed genes or of proteins and their interaction patterns will require enhanced performance to improve specificity of detection and dynamic range. To further extend the utility of DNA microarray-based approaches we present a high-performance tag microarray procedure that enables probe-based analysis of as little as 100 target cDNA molecules, and with a linear dynamic range close to 10(5). Furthermore, the protocol radically decreases the risk of cross-hybridization on microarrays compared to current approaches, and it also allows for quantification by single-molecule analysis and real-time on-chip monitoring of rolling-circle amplification. We provide proof of concept for microarray-based measurement of both mRNA molecules and of proteins, converted to tag DNA sequences by padlock and proximity probe ligation, respectively.

Subject headings and genre

  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • Jarvius, JonasUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)jonajarv (author)
  • Schallmeiner, EdithUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)edsch087 (author)
  • Howell, MAthiasUppsala universitet,Institutionen för genetik och patologi (author)
  • Nong, RachelUppsala universitet,Institutionen för genetik och patologi (author)
  • Reuter, Hendrik (author)
  • Hahn, Meinhard (author)
  • Stenberg, JohanUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)joste021 (author)
  • Nilsson, MatsUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)matsnils (author)
  • Landegren, UlfUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)ulfland (author)
  • Uppsala universitetInstitutionen för genetik och patologi (creator_code:org_t)

Related titles

  • In:Nucleic Acids Research: Oxford University Press (OUP)36:8, s. e45-0305-10481362-4962

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