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Neurosteroids alter...
Neurosteroids alter glutamate-induced changes in neurite morphology of NG108-15 cells
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- Johansson, Tobias (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Elfverson, Martin (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Birgner, Carolina (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Frändberg, Per-Anders (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Nyberg, Fred (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Le Grevès, Pierre (author)
- Uppsala universitet,Institutionen för neurovetenskap
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Journal of Steroid Biochemistry and Molecular Biology. - : Elsevier BV. - 0960-0760 .- 1879-1220. ; 104:3-5, s. 215-219
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Activation of the NMDA receptor leads to increased intracellular Ca2+ levels ([Ca2+]i) which induces outgrowth of and morphologic changes in the neurites of the NG108-15 cell line. This effect can be blocked by antagonists for this glutamate receptor subtype (e.g. ifenprodil or AP5). We have previously shown that nanomolar concentrations of various neurosteroids modulate ifenprodil binding to the NMDA receptor. To investigate whether this interaction affects the functioning of the receptor, we studied the effect of 24 and 48 h of pregnenolone sulphate (PS) or pregnanolone sulphate (3α5βS) on glutamate-stimulated NG108-15 cells. Unexpectedly, the neurosteroids themselves had an inhibitory effect on glutamate-induced changes in neurite patterns. This effect was comparable to that of ifenprodil or AP5. Moreover, the effect of combined treatment with 3α5βS and ifenprodil on neurite morphology indicated a functional interaction between the substances. Interestingly, PS induced cell detachment over time, an effect that was further enhanced by ifenprodil. Cell detachment was also seen after 48 h of treatment with 3α5βS; however, the effect was blocked by ifenprodil and weaker than that of PS. The interaction with the NR2B-selective antagonist ifenprodil indicates that this NMDA receptor subunit may be involved in neurosteroid-induced NG108-15 cell detachment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- NMDA receptor
- NR2B subunit
- Neurosteroids
- Ifenprodil
- Pregnenolone sulphate (PS)
- Pregnanolone sulphate (3α5βS)
- NG108-15
- Neurite outgrowth
- Cell adhesion
- PHARMACY
- FARMACI
Publication and Content Type
- ref (subject category)
- art (subject category)
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