Sökning: WFRF:(Caldwell Karin D.)
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Surface analysis of...
Surface analysis of pure and complex mucin coatings
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- Sandberg, Tomas (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Mellin, Lisa (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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- Gelius, Ulrik (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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visa fler...
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- Caldwell, Karin D. (författare)
- Uppsala universitet,Institutionen för fysikalisk och analytisk kemi
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visa färre...
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- Engelska.
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Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797 .- 1095-7103. ; 333:1, s. 180-187
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In the past, we introduced the idea of using mucin coatings to improve biomaterials performance. Here, we evaluate non-radioactive methods for the analysis of pure and human host protein-containing (complex) mucin coatings on a real-type substrate (Thermanox). A common protein quantification assay (mBCA) was combined with mass-calibrated, enzyme-amplified assays based on lectin (ELLA) and antibody (ELISA) recognition, to determine the total and specific amounts of surface-associated proteins. Model studies showed the mBCA assay to be of limited use at low mass loads, and steric effects to influence the ELLA at high surface layer densities. Non-specific responses due to substrate interaction were low for the ELLA and ELISAs. Cross-reactions were observed during ELLA analysis of analytes sharing high degree of O-glycosylation. Combined mBCA-ELLA-ELISA analysis suggested that mucin desorption was low upon protein addition and that low concentrations of ELISA-determined Protein for the complex coatings Could be explained in terms of low accessibility of proteins to the bulk environment. Specifically, a methodology is presented for the determination of the fraction of surface-exposed, presumed bioactive proteins in a complex mucin coating. Finally, X-ray photoelectron spectroscopy and infrared reflectance spectroscopy combined with multivariate data analysis were proven useful in the evaluation of mucin-based coatings.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Mucin quantification
- mBCA
- ELLA
- ELISA
- Ellipsometry
- Jacalin
- Complex mucin coating
- Surface-exposed protein
- Bioactivity
- XPS
- IRS-MVDA
- Principal component analysis (PCA)
- Chemistry
- Kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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