Potential use of mucins as biomaterial coatings. II. Mucin coatings affect the conformation and neutrophil-activating properties of adsorbed host proteins – Towards a mucosal mimic

• In continuation of our recent fractionation and characterization study   on mucins of bovine salivary (BSM), porcine gastric (PGM), and human   salivary (MG1) origin, this study evaluates the effect of mucin   precoating on the conformation and neutrophil-activating properties of   host proteins adsorbed to a polyethylene terephthalate-based model   biomaterial. Microscopy combined with assays for the neutrophil   releases of reactive oxygen species and human neutrophil lipocalin   showed that mucin precoating greatly reduced the strong immune-response   normally induced by adsorbed immunoglobulin G (IgG) and secretory   immunoglobulin A (sIgA), respectively. A similar finding was made for   the proinflammatory fibrinogen. Although the total uptakes of these   proteins depended on the mucin surface concentration, a detailed   composite analysis suggested the fraction Of surface-exposed protein to   be a stronger determinant of coating performance. The unexpectedly low   neutrophil activation showed by composites containing near-monolayer   concentrations of exposed IgG and sIgA, respectively, suggested that   these act synergistically with mucin on the surface. In support of this   hypothesis, quartz crystal microbalance with dissipation monitoring   measurements revealed that a preadsorbed BSM layer stabilizes IgG   through complexation on a polymeric model surface. Our findings link   well to the complex in vivo situation and suggest that functional   mucosal mimics can be created in situ for improved biomaterials   performance.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

mucin-based surface coatings

human neutrophil lipocalin (HNL)

surface-exposed protein

protein stabilization

quartz crystalmicrobalance with dissipation monitoring (QCM-D)

Chemistry

Kemi

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