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Delayed contraction...
Delayed contraction of the CD8+ T cell response toward lymphocytic choriomeningitis virus infection in mice lacking serglycin
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Grujic, Mirjana (författare)
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Christensen, Jan P. (författare)
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Sørensen, Maria R. (författare)
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visa fler...
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- Åbrink, Magnus (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Pejler, Gunnar (författare)
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Thomsen, Allan R. (författare)
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visa färre...
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(creator_code:org_t)
- 2008
- 2008
- Engelska.
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Ingår i: Journal of Immunology. - 0022-1767 .- 1550-6606. ; 181:2, s. 1043-1051
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We previously reported that the lack of serglycin proteoglycan affects secretory granule morphology and granzyme B (GrB) storage in in vitro generated CTLs. In this study, the role of serglycin during viral infection was studied by infecting wild-type (wt) mice and serglycin-deficient (SG(-/-)) mice with lymphocytic choriomeningitis virus (LCMV). Wt and SG(-/-) mice cleared 10(3) PFU of highly invasive LCMV with the same kinetics, and the CD8(+) T lymphocytes from wt and SG(-/-) animals did not differ in GrB, perforin, IFN-gamma, or TNF-alpha content. However, when a less invasive LCMV strain was used, SG(-/-) GrB(+) CD8(+) T cells contained approximately 30% less GrB than wt GrB(+) CD8(+) T cells. Interestingly, the contraction of the antiviral CD8(+) T cell response to highly invasive LCMV was markedly delayed in SG(-/-) mice, and a delayed contraction of the virus-specific CD8(+) T cell response was also seen after infection with vesicular stomatitis virus. BrdU labeling of cells in vivo revealed that the delayed contraction was associated with sustained proliferation of Ag-specific CD8(+) T cells in SG(-/-) mice. Moreover, wt LCMV-specific CD8(+) T cells from TCR318 transgenic mice expanded much more extensively in virus-infected SG(-/-) mice than in matched wt mice, indicating that the delayed contraction represents a T cell extrinsic phenomenon. In summary, the present report points to a novel, previously unrecognized role for serglycin proteoglycan in regulating the kinetics of antiviral CD8(+) T cell responses.
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- MEDICINE
- MEDICIN
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