Immunoreactivity against Goblet cells in patients with inflammatory bowel disease.

• BACKGROUND: A number of autoantibodies have been reported in inflammatory bowel disease (IBD). The aim of this study was to investigate to what extent sera from patients with IBD contain autoantibodies directed against normal human gastrointestinal mucosa. METHODS: Samples of sera from 50 patients with IBD and 50 healthy subjects were used for immunostaining of normal and affected human gastrointestinal tissues. RESULTS: Eighty-four percent of the sera from IBD patients showed immunoreactivity against goblet cells in the appendix compared with 8% of the sera from healthy subjects. Goblet cell reactivity of IBD patient sera varied between regions in the gastrointestinal tract. Sera from healthy subjects only reacted with goblet cells in the appendix. In the colon and the appendix, goblet cell reactivity of IBD sera was generally weak at the base of the crypts and gradually increased toward the lumen. Three IBD sera samples reacted with gastrin cells in the antrum. In colon biopsies from patients with ulcerative colitis, immunoreactivity against the remaining goblet cells showed an inverse correlation with inflammatory activity. CONCLUSIONS: These findings suggest that immunoreactivity against goblet cells may be of central importance in the pathogenesis of IBD. Identification of goblet cell antigens could lead to a better understanding of IBD and provide a new diagnostic tool.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Gastroenterologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)

Nyckelord

Adult

Aged

Antibodies

blood

immunology

Appendix

immunology

metabolism

pathology

Biopsy

Colitis

Ulcerative

immunology

metabolism

pathology

Colon

immunology

metabolism

pathology

Crohn Disease

immunology

metabolism

pathology

Duodenum

immunology

metabolism

pathology

Endoscopy

Gastrointestinal

Female

Goblet Cells

immunology

pathology

Humans

Immunity

Cellular

immunology

Immunohistochemistry

Inflammatory Bowel Diseases

immunology

metabolism

pathology

Male

Microscopy

Fluorescence

Middle Aged

Pyloric Antrum

immunology

metabolism

pathology

Severity of Illness Index

inflammatory bowel disease

MEDICINE

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