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Quantification of U...
Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and pPROM and its relation to inflammatory cytokine levels
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- Jacobsson, Bo, 1960 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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Aaltonen, R. (författare)
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Rantakokko-Jalava, K. (författare)
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visa fler...
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Morken, Nils-Halvdan, 1969 (författare)
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Alanen, A. (författare)
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visa färre...
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(creator_code:org_t)
- 2009
- 2009
- Engelska.
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Ingår i: Acta Obstet Gynecol Scand. ; 88:1, s. 63-70
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- OBJECTIVE: To study the effect of the amniotic fluid quantity of Ureaplasma urealyticum DNA on inflammatory response levels in women with preterm labor (PTL) and preterm prelabor rupture of membranes (pPROM). DESIGN: A prospective multi-center follow up study. SETTING: Sahlgrenska University Hospital, Goteborg, Sweden and Turku University Hospital, Turku, Finland. SAMPLE: Eleven U. urealyticum positive samples obtained after transabdominal amniocenteses in 197 women presenting with PTL and pPROM. METHODS: The U. urealyticum positive samples were analyzed with real-time polymerase chain reaction, using the Lightcycler instrument with primers specific for U. urealyticum 16 S rDNA. The amniotic fluid samples were analyzed for tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-1beta and IL-10 with enzyme-linked immunosorbent assays. MAIN OUTCOME MEASURES: Correlation between U. urealyticum DNA concentrations in the amniotic fluid and inflammatory cytokine levels. RESULTS: The concentrations of U. urealyticum DNA varied between 0.024 and 934 microg/mL. A significant correlation between U. urealyticum DNA and TNF-alpha level was observed. No correlation with the other cytokines was found. Women with PTLhad higher levels of U. urealyticum DNA and a different cytokine pattern than women with pPROM. CONCLUSIONS: U. urealyticum in the amniotic fluid induces an inflammatory reaction in a dose dependent manner and the quantity of U. urealyticum DNA is well correlated with the level of the inflammatory cytokine TNF-alpha.
Nyckelord
- Adult
- Amniocentesis
- Amniotic Fluid/chemistry
- Cytokines/analysis/*metabolism
- DNA
- Bacterial/*analysis
- Enzyme-Linked Immunosorbent Assay
- Female
- Fetal Membranes
- Premature Rupture/*diagnosis/etiology
- Finland
- Follow-Up Studies
- Gestational Age
- Humans
- Inflammation Mediators/*analysis
- Obstetric Labor
- Premature/*diagnosis/etiology/microbiology
- Polymerase Chain Reaction
- Predictive Value of Tests
- Pregnancy
- Pregnancy Outcome
- Prospective Studies
- Risk Assessment
- Sensitivity and Specificity
- Sweden
- Ureaplasma/isolation & purification
- Ureaplasma Infections/complications/*diagnosis
- Young Adult
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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