Steady-state migrating intestinal dendritic cells induce potent inflammatory responses in naive CD4+ T cells.

• Steady-state dendritic cells (DCs) migrating in the lymph from the intestine induce tolerance to harmless intestinal antigens, preventing inflammatory responses. To determine if such DCs are inherently tolerogenic we collected intestinal lymph DCs (L-DCs) by cannulation of the thoracic duct of rats after mesenteric lymphadenectomy, and examined their capacity to activate naive CD4+ lymphocytes in an allogeneic mixed leucocyte reaction. L-DCs stimulated strong proliferative responses, induced secretion of inflammatory cytokines including interferon-gamma, and induced FoxP3-positive lymphocytes to divide. To determine if the activated CD4+ T cells had been tolerized, they were rested and restimulated with irradiated splenocytes. The restimulated CD4+ T cells again proliferated and secreted inflammatory cytokines. These data demonstrate that the DCs, which migrate from the intestine in the steady state, are paradoxically able to induce strong inflammatory responses from naive T cells, despite their role in the maintenance of oral tolerance.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Keyword

Animals

CD4-Positive T-Lymphocytes

immunology

Cell Movement

physiology

Cell Proliferation

Cells

Cultured

Cytokines

secretion

Dendritic Cells

immunology

physiology

Inflammation

immunology

Interferon-gamma

immunology

Lymph

immunology

Lymph Node Excision

methods

Lymphocyte Activation

immunology

Lymphocyte Culture Test

Mixed

methods

Lymphocytes

immunology

Male

Mesentery

immunology

surgery

Rats

Th1 Cells

immunology

Publication and Content Type

ref (subject category)

art (subject category)