Steady-state migrating intestinal dendritic cells induce potent inflammatory responses in naive CD4+ T cells.

Steady-state dendritic cells (DCs) migrating in the lymph from the intestine induce tolerance to harmless intestinal antigens, preventing inflammatory responses. To determine if such DCs are inherently tolerogenic we collected intestinal lymph DCs (L-DCs) by cannulation of the thoracic duct of rats after mesenteric lymphadenectomy, and examined their capacity to activate naive CD4+ lymphocytes in an allogeneic mixed leucocyte reaction. L-DCs stimulated strong proliferative responses, induced secretion of inflammatory cytokines including interferon-gamma, and induced FoxP3-positive lymphocytes to divide. To determine if the activated CD4+ T cells had been tolerized, they were rested and restimulated with irradiated splenocytes. The restimulated CD4+ T cells again proliferated and secreted inflammatory cytokines. These data demonstrate that the DCs, which migrate from the intestine in the steady state, are paradoxically able to induce strong inflammatory responses from naive T cells, despite their role in the maintenance of oral tolerance.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
Animals
CD4-Positive T-Lymphocytes
immunology
Cell Movement
physiology
Cell Proliferation
Cells
Cultured
Cytokines
secretion
Dendritic Cells
immunology
physiology
Inflammation
immunology
Interferon-gamma
immunology
Lymph
immunology
Lymph Node Excision
methods
Lymphocyte Activation
immunology
Lymphocyte Culture Test
Mixed
methods
Lymphocytes
immunology
Male
Mesentery
immunology
surgery
Rats
Th1 Cells
immunology

Jenkins, C D (författare)
Yrlid, Ulf,1971Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xyrlul (författare)
Cerovic, V (författare)
Edmond, H (författare)
McDonald, V (författare)
Nassar, M (författare)
Macpherson, G (författare)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi (creator_code:org_t)

Av författaren/redakt...: Milling, S W F; Jenkins, C D; Yrlid, Ulf, 1971; Cerovic, V; Edmond, H; McDonald, V; visa fler...; Nassar, M; Macpherson, G; visa färre...

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