Gastric mucosal recognition of Helicobacter pylori is independent of Toll-like receptor 4

Bäckhed, Fredrik,1973Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory (författare)

Little is known about the interactions between Helicobacter pylori, which specializes in colonizing the mucin layer that covers the gastric mucosa, and primary gastric epithelial cells. The expression pattern of Toll-like receptors (TLRs) in primary gastric epithelial cells and cell lines was compared. Primary cells did not express TLR4, whereas all cell lines expressed a nonsignaling form of TLR4. Because other cells within the mucosa expressed TLR4, it was next investigated whether H. pylori can be recognized by TLR4--they cannot. Moreover, H. pylori infection of primary cells induced a regulated production of interleukin (IL)-6, IL-8, and tumor necrosis factor-alpha, whereas infection of cell lines only resulted in IL-8 production. The cytokine production in all cell types was strictly cag dependent. These findings indicate that, although the epithelium is important in directing the immune response against H. pylori infections, the response is independent of TLR4.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
Adolescent
Adult
Biopsy
Cells
Cultured
Drosophila Proteins
Epithelial Cells/immunology/microbiology
Female
Gastric Mucosa/ microbiology
Helicobacter pylori/ pathogenicity
Humans
Male
Membrane Glycoproteins/ metabolism
Middle Aged
Pyloric Antrum/cytology/immunology/microbiology
Receptors
Cell Surface/ metabolism
Toll-Like Receptor 4
Toll-Like Receptors
Tumor Cells
Cultured

Av författaren/redakt...: Bäckhed, Fredrik ...; Rokbi, B.; Torstensson, E.; Zhao, Y.; Nilsson, C.; Seguin, D.; visa fler...; Normark, S.; Buchan, A. M.; Richter-Dahlfors ...; visa färre...

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