Cytokine responses of human gingival fibroblasts to Actinobacillus actinomycetemcomitans cytolethal distending toxin.

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: id:"swepub:oai:gup.ub.gu.se/106093" > Cytokine responses ...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Belibasakis, G NUmeå universitet,Oral mikrobiologi,Oral cellbiologi (author)

Cytokine responses of human gingival fibroblasts to Actinobacillus actinomycetemcomitans cytolethal distending toxin.

Article/chapterEnglish2005

Publisher, publication year, extent ...

Elsevier BV,2005

Numbers

LIBRIS-ID:oai:gup.ub.gu.se/106093
https://gup.ub.gu.se/publication/106093URI
https://doi.org/10.1016/j.cyto.2004.11.008DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-18083URI

Supplementary language notes

Language:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Actinobacillus actinomycetemcomitans is implicated in the pathogenesis of localized aggressive periodontitis, and has the capacity to express a cytolethal distending toxin (Cdt). Gingival fibroblasts (GF) are resident cells of the periodontium, which can express several osteolytic cytokines. The aims of this study were a) to investigate the role of Cdt in A. actinomycetemcomitans-induced expression of osteolytic cytokines and their cognate receptors in GF and b) to determine if the previously demonstrated induction of receptor activator of NFkappaB ligand (RANKL) by A. actinomycetemcomitans is mediated by these pro-inflammatory cytokines or by prostaglandin E(2) (PGE(2)). A. actinomycetemcomitans clearly induced interleukin (IL)-6, IL-1beta, and to a minimal extent, tumor necrosis factor (TNF)-alpha mRNA expression. At the protein level, IL-6 but not IL-1beta or TNF-alpha expression was stimulated. The mRNA expression of the different receptor subtypes recognizing IL-6, IL-1beta and TNF-alpha was not affected. A cdt-knockout strain of A. actinomycetemcomitans had similar effects on cytokine and cytokine receptor mRNA expression, compared to its parental wild-type strain. Purified Cdt stimulated IL-6, but not IL-1beta or TNF-alpha protein biosynthesis. Antibodies neutralizing IL-6, IL-1 or TNF-alpha, and the PGE(2) synthesis inhibitor indomethacin, did not affect A. actinomycetemcomitans-induced RANKL expression. In conclusion, a) A. actinomycetemcomitans induces IL-6 production in GF by a mechanism largely independent of its Cdt and b) A. actinomycetemcomitans-induced RANKL expression in GF occurs independently of IL-1, IL-6, TNF-alpha, or PGE(2).

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Fysiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Physiology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Odontologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dentistry hsv//eng
Actinobacillus actinomycetemcomitans
genetics
pathogenicity
Bacterial Toxins
toxicity
Carrier Proteins
drug effects
genetics
metabolism
Cytokines
drug effects
genetics
metabolism
Dinoprostone
metabolism
Fibroblasts
drug effects
immunology
Gene Expression Regulation
drug effects
Gingiva
drug effects
metabolism
microbiology
Humans
Indomethacin
pharmacology
Inflammation Mediators
immunology
metabolism
Interleukin 1 Receptor Antagonist Protein
Membrane Glycoproteins
drug effects
genetics
metabolism
Mutation
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Receptors
Interleukin-1
drug effects
genetics
metabolism
Receptors
Interleukin-1 Type II
Receptors
Interleukin-6
drug effects
genetics
metabolism
Receptors
Tumor Necrosis Factor
Type I
drug effects
genetics
metabolism
Receptors
Tumor Necrosis Factor
Type II
drug effects
genetics
metabolism
Sialoglycoproteins
pharmacology

Added entries (persons, corporate bodies, meetings, titles ...)

Johansson, AndersUmeå universitet,Parodontologi(Swepub:umu)anjo0002 (author)
Wang, Y (author)
Chen, C (author)
Lagergård, Teresa,1946Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institute of Medical Microbiology/Immunology(Swepub:gu)xlagte (author)
Kalfas, S (author)
Lerner, Ulf HUmeå universitet,Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine,Oral cellbiologi(Swepub:umu)ulle0001 (author)
Umeå universitetOral mikrobiologi (creator_code:org_t)

Related titles

In:Cytokine: Elsevier BV30:2, s. 56-631043-46661096-0023

Internet link

Find in a library

Cytokine (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Belibasakis, G N; Johansson, Ander ...; Wang, Y; Chen, C; Lagergård, Teres ...; Kalfas, S; show more...; Lerner, Ulf H; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Endocrinology an ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dentistry

Articles in the publication: Cytokine

By the university: University of Gothenburg; Umeå University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se