Family 2 cystatins inhibit osteoclast-mediated bone resorption in calvarial bone explants.

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Lerner Ulf) > (2000-2004) > Family 2 cystatins ...

Details
MARC

Brand, H S (author)

Family 2 cystatins inhibit osteoclast-mediated bone resorption in calvarial bone explants.

Article/chapterEnglish2004

Publisher, publication year, extent ...

Elsevier BV,2004

Numbers

LIBRIS-ID:oai:gup.ub.gu.se/106100
https://gup.ub.gu.se/publication/106100URI
https://doi.org/10.1016/j.bone.2004.05.015DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-18057URI
https://lup.lub.lu.se/record/266572URI

Supplementary language notes

Language:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Osteoclastic bone resorption depends on the activity of various proteolytic enzymes, in particular those belonging to the group of cysteine proteinases. Biochemical studies have shown that cystatins, naturally occurring inhibitors of these enzymes, inhibit bone matrix degradation. Since the mechanism by which cystatins exert this inhibitory effect is not completely resolved yet, we studied the effect of cystatins on bone resorption microscopically and by Ca-release measurements. Calvarial bone explants were cultured in the presence or absence of family 2 cystatins and processed for light and electron microscopic analysis, and the culture media were analyzed for calcium release. Both egg white cystatin and human cystatin C decreased calcium release into the medium significantly. Microscopic analyses of the bone explants demonstrated that in the presence of either inhibitor, a high percentage of osteoclasts was associated with demineralized non-degraded bone matrix. Following a 24-h incubation in the presence of cystatin C, 41% of the cells were adjacent to areas of demineralized non-degraded bone matrix, whereas in controls, this was only 6%. If bone explants were cultured with both PTH and cystatin C, 60% of the osteoclasts were associated with demineralized non-degraded bone matrix, compared to 27% for bones treated with PTH only (P < 0.01). Our study provides evidence that cystatins, the naturally occurring inhibitors of cysteine proteinases, reversibly inhibit bone matrix degradation in the resorption lacunae adjacent to osteoclasts. These findings suggest the involvement of cystatins in the modulation of osteoclastic bone degradation.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Odontologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dentistry hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Fysiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Physiology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Ortopedi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Orthopaedics hsv//eng
Animals
Bone Resorption
metabolism
prevention & control
Cattle
Cells
Cultured
Chickens
Cystatin C
Cystatins
pharmacology
Dose-Response Relationship
Drug
Humans
Mice
Osteoclasts
drug effects
metabolism
ultrastructure
Skull
drug effects
physiology
ultrastructure
cysteine proteinases
cystatins
cathepsins
bone
calcium
osteoclasts

Added entries (persons, corporate bodies, meetings, titles ...)

Lerner, Ulf HUmeå universitet,Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine,Oral cellbiologi(Swepub:umu)ulle0001 (author)
Grubb, AndersLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kkem-agr (author)
Beertsen, W (author)
Nieuw Amerongen, A V (author)
Everts, V (author)
Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för internmedicin (creator_code:org_t)

Related titles

In:Bone: Elsevier BV35:3, s. 689-968756-32821873-2763

Internet link

Find in a library

Bone (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Brand, H S; Lerner, Ulf H; Grubb, Anders; Beertsen, W; Nieuw Amerongen, ...; Everts, V

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dentistry

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Endocrinology an ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Orthopaedics

Articles in the publication: Bone

By the university: University of Gothenburg; Umeå University; Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se