Glucocorticoid receptor inhibits microtubule assembly in vitro.

• The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein M(r) 90,000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Animals

Blotting

Western

Cattle

HSP90 Heat-Shock Proteins

physiology

Kinetics

Microscopy

Electron

Microtubule Proteins

metabolism

Microtubules

metabolism

ultrastructure

Rats

Receptors

Glucocorticoid

physiology

Spectrophotometry

Cellforskning

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)