Dynamic instability of microtubules from cold-living fishes.

Billger, MartinGothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology (author)

The dynamic instability of microtubules free of microtubule-associated proteins from two genera of cold-living fishes was measured, by means of video-enhanced differential-interference-contrast microscopy, at temperatures near those of their habitats. Brain microtubules were isolated from the boreal Atlantic cod (Gadus morhua; habitat temperature approximately 2-15 degrees C) and from two austral Antarctic rockcods (Notothenia gibberifrons and N. coriiceps neglecta; habitat temperature approximately -1.8 to + 2 degrees C). Critical concentrations for polymerization of the fish tubulins were in the neighborhood of 1 mg/ml, consistent with high interdimer affinities. Rates of elongation and frequencies of growth-to-shortening transitions ("catastrophes") for fish microtubules were significantly smaller than those for mammalian microtubules. Slow dynamics is therefore an intrinsic property of these fish tubulins, presumably reflecting their adaptation to low temperatures. Two-dimensional electrophoresis showed striking differences between the isoform compositions of the cod and the rockcod tubulins, which suggests that the cold-adapted microtubule phenotypes of northern and southern fishes may have arisen independently.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Animals
Brain
physiology
Cold Temperature
Electrophoresis
Gel
Two-Dimensional
Fishes
physiology
Microscopy
Interference
methods
Microscopy
Video
Microtubules
physiology
Tubulin
chemistry

Wallin, Margareta,1952Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology(Swepub:gu)xwmars (author)
Williams, R C (author)
Detrich, H W (author)
Göteborgs universitetZoologiska institutionen (creator_code:org_t)

By the author/editor: Billger, Martin; Wallin, Margaret ...; Williams, R C; Detrich, H W

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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