The leukocyte antigen CD43 is expressed in different cell lines of nonhematopoietic origin.

• CD43 is an abundant transmembrane sialoglycoprotein in leukocyte-type cell lines, but it has also been suggested to be present in colon adenomas and colon carcinomas. We have now shown that CD43 is expressed in a variety of cell lines of different origins (CaSKI, A549, 293, MTSV1-7, MCF7, HT-1080, Jurkat, K562, COLO 205, HT-29, Caco-2, DLD-1 and SW480). The level of expression of CD43 mRNA was analyzed by reverse transcriptase-polymerase chain reaction and that of the protein by immunoprecipitation and Western blot, flow cytometry and confocal microscopy using two monoclonal anti-CD43 antibodies (L10 and 4D2). As all cell lines expressed CD43, it is suggested that CD43 has a more fundamental function than previously believed and thus cannot be considered only as a specific leukocyte marker.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Nyckelord

Antibodies

Monoclonal

metabolism

Antigens

CD

Antigens

CD43

Blotting

Western

Cell Line

Cell Separation

Electrophoresis

Polyacrylamide Gel

Exons

Flow Cytometry

Humans

Introns

Jurkat Cells

Microscopy

Confocal

Microscopy

Fluorescence

Plasmids

metabolism

Precipitin Tests

Reverse Transcriptase Polymerase Chain Reaction

Sialoglycoproteins

biosynthesis

metabolism

Tumor Cells

Cultured

Publikations- och innehållstyp

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