IL-5 expression and release from human CD34 cells in vitro; ex vivo evidence from cases of asthma and Churg-Strauss syndrome.

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Bossios, Apostolos,1969Gothenburg University,Göteborgs universitet,Krefting Research Centre (author)

IL-5 expression and release from human CD34 cells in vitro; ex vivo evidence from cases of asthma and Churg-Strauss syndrome.

Article/chapterEnglish2009

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2009-11-26
Wiley,2009

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LIBRIS-ID:oai:gup.ub.gu.se/106682
https://gup.ub.gu.se/publication/106682URI
https://doi.org/10.1111/j.1398-9995.2009.02271.xDOI

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Language:English

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Subject category:art swepub-publicationtype

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To cite this article: Bossios A, Sjöstrand M, Dahlborn A-K, Samitas K, Malmhäll C, Gaga M, Lötvall J. IL-5 expression and release from human CD34 cells in vitro; ex vivo evidence from cases of asthma and Churg-Strauss syndrome. Allergy 2009. DOI: 10.1111/j.1398-9995.2009.02271.x.Abstract Background: Eosinophils develop from hematopoietic CD34(+) progenitor cells in the bone marrow (BM) under the influence of Interleukin-5 (IL-5). The primary source of IL-5 is T-lymphocytes, although other sources may exist. The aims of this study were to determine whether CD34(+) cells from human peripheral blood (PB) and BM have the capacity to produce IL-5 when stimulated in vitro, and secondly, whether an elevated number of IL-5-producing CD34(+) cells can be found in situ in ongoing eosinophilic disease. Methods: CD34(+) cells from PB and BM were stimulated in vitro, and IL-5 production and release was assessed by ELISA, ELISPOT, flow cytometry and immunocytochemistry. Blood and BM from a patient with Churg-Strauss syndrome were analyzed by flow cytometry for CD34(+)/IL-5(+) cells, and immunohistochemical staining of CD34(+)/IL-5(+) cells in bronchial biopsies from an asthmatic patient was performed. Results: Both PB and BM CD34(+) cells can produce and release IL-5 when stimulated in vitro. In the Churg-Strauss patient, IL-5-producing CD34(+) cells were found in PB and BM. Oral glucocorticoid treatment markedly decreased the number of IL-5-positive CD34 cells in the BM. CD34(+)/IL-5(+) cells were present in a patient with asthma. Conclusion: CD34(+) cells in blood and BM are capable of producing IL-5 both in vitro and in vivo in humans, arguing that these cells may have the capacity to contribute to eosinophilic inflammation. Consequently, targeting CD34(+) progenitor cells that produce and release IL-5 may be effective in reducing the mobilization of eosinophil lineage-committed cells in eosinophilic-driven diseases.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Lungmedicin och allergi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Respiratory Medicine and Allergy hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
IL-5
CD34
Asthma
Churg-Strauss

Added entries (persons, corporate bodies, meetings, titles ...)

Sjöstrand, Margareta,1947Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xsjosm (author)
Dahlborn, A-KGothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine (author)
Samitas, Konstantinos,1977Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xsamko (author)
Malmhäll, Carina,1959Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xmalmc (author)
Gaga, M (author)
Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xlotja (author)
Göteborgs universitetKrefting Research Centre (creator_code:org_t)

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In:Allergy: Wiley:Nov 261398-99950105-4538

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