Sökning: WFRF:(Jonsson Charlotte A 1971)
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Accumulation of FIT...
Accumulation of FITC near stratum corneum-visualizing epidermal distribution of a strong sensitizer using two-photon microscopy
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- Samuelsson, Kristin, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Simonsson, Carl, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Jonsson, Charlotte A, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Westman, Gunnar, 1964 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ericson, Marica B, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institutionen för fysik (GU),Institute of Clinical Sciences,Department of Physics (GU),University of Gothenburg
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- Karlberg, Ann-Therese, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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(creator_code:org_t)
- Wiley, 2009
- 2009
- Engelska.
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Ingår i: Contact Dermatitis. - : Wiley. - 0105-1873 .- 1600-0536. ; 61:2, s. 91-100
- Relaterad länk:
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Background The allergenic potency of a hapten is related to its skin penetration properties, but little is known about the distribution of haptens in the skin following topical application. Objectives The aim of this study was to investigate the diffusion and epidermal distribution using two-photon microscopy (TPM) of two fluorescent compounds. Methods Sensitizing capacities of fluorescein isothiocyanate (FITC) and fluorescein were investigated using the local lymph node assay. Chemical reactivity of the compounds was analysed, and their distribution in human epidermis was visualized using TPM and confocal microscopy. Also the in vitro diffusion through epidermis of FITC and fluorescein has been examined. Results FITC was classified as an extreme sensitizer, whereas fluorescein was non-sensitizing. TPM and confocal microscopy showed an accumulation of FITC in stratum corneum (SC), whereas fluorescein was more evenly distributed in epidermis. The diffusion of fluorescein through epidermis was three times higher than that of FITC. Conclusions TPM, which has never been used in this context before, is a promising tool for visualizing the distribution of fluorescent compounds of varying reactivity in intact skin. The strong allergen FITC is mainly retained in or adjacent to SC, whereas most fluorescein diffused through the epidermis.
Ämnesord
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- contact allergy
- FITC
- fluorescent hapten
- laser scanning confocal
- microscopy
- local lymph node assay
- skin penetration
- two-photon
- microscopy
- lymph-node assay
- allergic contact-dermatitis
- in-vitro
- skin
- sensitization
- peptide reactivity
- fluorescein isothiocyanate
- classification
- dinitrochlorobenzene
- phenylisothiocyanate
- hypersensitivity
- local lymph node assay
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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