Woodsholea maritima gen. nov., sp. nov., a marine bacterium with a low diversity of polar lipids.

• Two cauliform bacteria (CM243T and CM251) isolated by J. Poindexter from the Atlantic Ocean were characterized by 16S rRNA gene sequencing, TaqI restriction fragment length polymorphism and single-strand conformation polymorphism analyses of the internally transcribed 16S-23S rDNA spacer (ITS1) region, analysis of fatty acids from cellular lipids, mass spectrometry of polar lipids and physiological properties. The two strains showed very low diversity of polar lipids with diacyl-sulfoquinovosyl glycerols as the predominant lipids. The two bacterial strains were observed to have nearly identical 16S rRNA gene sequences and could not be differentiated by their ITS1 regions. The isolates differed from species of the genus Maricaulis by their 16S rRNA gene sequences, polar lipids and fatty acid patterns. On the basis of the genotypic analyses and estimations of phylogenetic similarities, physiological and chemotaxonomic characteristics, it is proposed that the isolates represent a new genus and species, for which the name Woodsholea maritima gen. nov., sp. nov. (type strain CM243T=VKM B-1512T=LMG 21817T) is proposed.

Subject headings

NATURVETENSKAP  -- Biologi -- Biologisk systematik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biological Systematics (hsv//eng)

Keyword

Atlantic Ocean

Bacterial Typing Techniques

Caulobacteraceae

classification

isolation & purification

physiology

ultrastructure

DNA

Bacterial

chemistry

DNA

Ribosomal

chemistry

DNA

Ribosomal Spacer

genetics

isolation & purification

Genes

rRNA

Lipids

analysis

isolation & purification

Microscopy

Microscopy

Electron

Molecular Sequence Data

Phylogeny

Polymorphism

Single-Stranded Conformational

RNA

Bacterial

genetics

RNA

Ribosomal

16S

genetics

Seawater

microbiology

Sequence Analysis

DNA

Sequence Homology

Water Microbiology

Publication and Content Type

ref (subject category)

art (subject category)