Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.

Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg(-1) creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
Adult
Chromatography
High Pressure Liquid
Creatinine
urine
Deoxyguanosine
analogs & derivatives
urine
Electrochemistry
Enzyme-Linked Immunosorbent Assay
Gas Chromatography-Mass Spectrometry
Humans
Laboratories
Male
Middle Aged

Barregård, Lars,1948Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine(Swepub:gu)xbarrl (author)
Mistry, Vilas (author)
Potdar, Neelam (author)
Rozalski, Rafal (author)
Gackowski, Daniel (author)
Siomek, Agnieszka (author)
Foksinski, Marek (author)
Svoboda, Peter (author)
Kasai, Hiroshi (author)
Konje, Justin C (author)
Sällsten, Gerd,1952Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine(Swepub:gu)xsalge (author)
Evans, Mark D (author)
Olinski, Ryszard (author)
Göteborgs universitetInstitutionen för medicin, avdelningen för samhällsmedicin och folkhälsa (creator_code:org_t)

In:Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals: Informa UK Limited14:2, s. 103-101366-5804
In:Biomarkers: Informa UK Limited14:2, s. 103-101354-750X

By the author/editor: Cooke, Marcus S; Barregård, Lars, ...; Mistry, Vilas; Potdar, Neelam; Rozalski, Rafal; Gackowski, Danie ...; show more...; Siomek, Agnieszk ...; Foksinski, Marek; Svoboda, Peter; Kasai, Hiroshi; Konje, Justin C; Sällsten, Gerd, ...; Evans, Mark D; Olinski, Ryszard; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dermatology and ...

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