A calmodulin inhibitor with high specificity, compound 48/80, inhibits axonal transport in frog nerves without disruption of axonal microtubules.

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Ekström, Per (författare)

A calmodulin inhibitor with high specificity, compound 48/80, inhibits axonal transport in frog nerves without disruption of axonal microtubules.

Artikel/kapitelEngelska1991

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1991

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LIBRIS-ID:oai:gup.ub.gu.se/114041
https://gup.ub.gu.se/publication/114041URI
https://lup.lub.lu.se/record/5fea66fd-c20a-486d-a6f5-4a49440db675URI

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Språk:engelska

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Ämneskategori:art swepub-publicationtype

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The calmodulin inhibitor compound 48/80 has previously been shown to arrest axonal transport in vitro in the regenerating frog sciatic nerve. The inhibition was limited to the outgrowth region of nerves, which had been allowed to regenerate in vivo for 6 days after a crush lesion, before they were incubated with or without drugs in vitro overnight. The effects of compound 48/80 on the regenerating nerve were further investigated. A concentration of compound 48/80 (50 micrograms ml-1), which effectively inhibits axonal transport, did not cause observable changes of the microtubules of regenerating axons in the outgrowth region as judged by electron microscopy. Furthermore, it was shown that also a lower concentration (25 micrograms ml-1) inhibited axonal transport. As a measure of possible metabolic effects, the level of ATP was assessed in the regenerating nerve after exposure to compound 48/80. Compound 48/80 at 25 micrograms ml-1 did not change the level of ATP in the nerve. The assembly of bovine brain microtubule proteins in a cell-free system was unaffected by 25 micrograms ml-1 of compound 48/80 and slightly inhibited by 50 micrograms ml-1. At higher concentrations (greater than 100 micrograms ml-1) assembly of microtubules appeared stimulated, and microtubule spirals as well as closely aligned microtubules could be seen. These effects appeared to be unrelated to the transport effects. The present results indicate that compound 48/80 arrests axonal transport via mechanisms other than destruction of axonal microtubules or interference with the energy metabolism. It is possible that these mechanisms involve inhibition of calmodulin-regulated events essential to the transport.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Cellbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Cell Biology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
Adenosine Triphosphate
metabolism
Animals
Axonal Transport
drug effects
physiology
Axons
metabolism
physiology
ultrastructure
Electrophoresis
Polyacrylamide Gel
Microscopy
Electron
Microtubules
metabolism
physiology
ultrastructure
Phosphoproteins
analysis
metabolism
Rana temporaria
p-Methoxy-N-methylphenethylamine
pharmacology
axonal transport
calmodulin
compound 48/80
frog sciatic nerve
microtubule proteins
microtubules

Biuppslag (personer, institutioner, konferenser, titlar ...)

Wallin, Margareta,1952Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology(Swepub:gu)xwmars (författare)
Kanje, M.Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)zoof-mka (författare)
Edström, AndersLund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science(Swepub:lu)zoof-aed (författare)
Wallin, Maria (författare)
Göteborgs universitetZoologiska institutionen (creator_code:org_t)

Sammanhörande titlar

Ingår i:Acta physiologica Scandinavica142:2, s. 181-90001-6772

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Av författaren/redakt...: Ekström, Per; Wallin, Margaret ...; Kanje, M.; Edström, Anders; Wallin, Maria

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Biokemi och mole ...

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Cellbiologi

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Neurovetenskaper

Artiklar i publikationen: Acta physiologic ...

Av lärosätet: Göteborgs universitet; Lunds universitet

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