Search: WFRF:(Thörn Ingrid)
> (2010-2014) >
Applicability of IG...
Applicability of IG/TCR gene rearrangements as targets for minimal residual disease assessment in a population-based cohort of Swedish childhood acute lymphoblastic leukaemia diagnosed 2002-2006.
-
- Thörn, Ingrid (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Forestier, Erik (author)
- Umeå universitet,Pediatrik
-
- Thuresson, Britt (author)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine
-
show more...
-
- Wasslavik, Carina (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
-
Malec, Maria (author)
-
- Li, Aihong (author)
- Umeå universitet,Klinisk kemi
-
Lindström-Eriksson, Elenor (author)
-
- Botling, Johan (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Barbany, Gisela (author)
- Karolinska Institutet,Uppsala universitet,Institutionen för genetik och patologi
-
- Jacobsson, Stefan, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
-
- Olofsson, Tor (author)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine
-
- Porwit, Anna (author)
- Karolinska Institutet
-
- Sundström, Christer (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
- Rosenquist, Richard (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
show less...
-
(creator_code:org_t)
- Wiley, 2010
- 2010
- English.
-
In: European journal of haematology. - : Wiley. - 1600-0609 .- 0902-4441. ; 84:2, s. 117-27
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://lup.lub.lu.s...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Minimal residual disease (MRD) detection during the early treatment phase has become an important stratification parameter in many childhood acute lymphoblastic leukaemia (ALL) treatment protocols. Here, we aimed to address the applicability of rearranged antigen-receptor genes as potential MRD markers using real-time quantitative polymerase chain reaction (RQ-PCR) in a Swedish population-based cohort. From 334 childhood ALL cases diagnosed during 2002-2006, we analysed 279 diagnostic samples (84%) by screening for rearranged immunoglobulin (IG) and T-cell receptor (TCR) genes. Allele-specific oligonucleotides were designed, and the sensitivity and quantitative level was determined for each target. Overall, clonal IG/TCR rearrangements were detected in 97% (236/244) of B-cell precursor ALL (BCP ALL) and 94% (33/35) of T-ALL. A sensitive RQ-PCR analysis (< or = 10(-4)) was obtained in 89% (216/244) of BCP ALL and in 74% (26/35) of T-ALL, whereas two sensitive targets were only available in 47% (115/244) of BCP ALL and 29% (10/35) of T-ALL cases. With the stratification threshold of > or = 10(-3), which is applied in the current Nordic treatment protocol (NOPHO-ALL 2008) for the identification of high-risk patients, 93% of BCP ALL and 86% of T-ALL reached this quantitative range by at least one target gene. Taken together, this national retrospective study demonstrates that an IG/TCR target for MRD monitoring can be identified in the majority of childhood ALL cases, whereas identification of a second sensitive target gene needs to be improved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Adolescent
- Alleles
- Antineoplastic Combined Chemotherapy Protocols
- administration & dosage
- Child
- Child
- Preschool
- Female
- Gene Rearrangement
- B-Lymphocyte
- Gene Rearrangement
- T-Lymphocyte
- Humans
- Infant
- Infant
- Newborn
- Male
- Monitoring
- Physiologic
- methods
- Neoplasm
- Residual
- Polymerase Chain Reaction
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
- drug therapy
- metabolism
- Retrospective Studies
- Sensitivity and Specificity
- Sweden
- minimal residual disease
- T-cell receptor genes
- immunoglobulin
- real-time quantitative polymerase chain reaction
- rearranged
- childhood acute lymphoblastic leukaemia
- real-time quantitative polymerase chain reaction
- MEDICINE
- medicin
- Haematology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Thörn, Ingrid
-
Forestier, Erik
-
Thuresson, Britt
-
Wasslavik, Carin ...
-
Malec, Maria
-
Li, Aihong
-
show more...
-
Lindström-Erikss ...
-
Botling, Johan
-
Barbany, Gisela
-
Jacobsson, Stefa ...
-
Olofsson, Tor
-
Porwit, Anna
-
Sundström, Chris ...
-
Rosenquist, Rich ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Hematology
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
European journal ...
- By the university
-
University of Gothenburg
-
Lund University
-
Umeå University
-
Uppsala University
-
Karolinska Institutet