Sökning: WFRF:(Magnusson Yvonne 1957) >
Functional analysis...
Functional analysis of rabbit anti-peptide antibodies which mimic autoantibodies against the beta 1-adrenergic receptor in patients with idiopathic dilated cardiomyopathy.
-
- Magnusson, Yvonne, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
-
Wallukat, G (författare)
-
Guillet, J G (författare)
-
visa fler...
-
- Hjalmarson, Agneta, 1943 (författare)
- Gothenburg University,Göteborgs universitet,Hjärt-kärlinstitutionen,Cardiovascular Institute
-
Hoebeke, Johan (författare)
-
visa färre...
-
(creator_code:org_t)
- 1991
- 1991
- Engelska.
-
Ingår i: Journal of autoimmunity. - 0896-8411. ; 4:6, s. 893-905
- Relaterad länk:
-
https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- A synthetic peptide corresponding to the second extracellular loop of the beta 1-adrenergic receptor was used as an antigen for antibody production in three rabbits. Antibodies of high titers were obtained in all rabbits. Only one rabbit yielded antibodies which decreased radioligand binding on the receptor in a similar way to that described for autoantibodies in patients with dilated cardiomyopathy. These antibodies recognized the receptor protein in immunoblots. Epitope mapping indicated that the N-terminal sequence of the loop used as antigen was the target of the major antigen fraction. Incubation of antibodies with C6 glioma cell membranes or inner membranes of E. coli, which express the human beta 1-adrenergic receptor, resulted in a decrease in number of radioligand binding sites. This decrease was dependent on the concentration of antibody and of Mg++ ions. It was not affected by the GTP analog GppNHp or the beta 1 subtype-specific antagonist metoprolol. The agonist, isoproterenol, also induced a decrease but the effects of antibody and agonist were not additive. These results suggest that the antibodies induce a Mg(++)-dependent, 'active', labile conformation of the receptor, independent from coupling to the GTP regulatory protein, but similar to that induced by the agonist isoproterenol. This interpretation was corroborated by the beta 1-adrenergic receptor agonist-like effect of the antibodies on cardiomyocytes in culture.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Amino Acid Sequence
- Animals
- Autoantibodies
- immunology
- Binding Sites
- drug effects
- Binding
- Competitive
- Cardiomyopathy
- Hypertrophic
- immunology
- Dose-Response Relationship
- Immunologic
- Glioma
- immunology
- Guanylyl Imidodiphosphate
- pharmacology
- Humans
- Iodocyanopindolol
- Isoproterenol
- pharmacology
- Magnesium
- Metoprolol
- pharmacology
- Molecular Sequence Data
- Pindolol
- analogs & derivatives
- Rabbits
- Radioligand Assay
- Receptors
- Adrenergic
- beta
- immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas