Glycosphingolipid receptors for Pseudomonas aeruginosa.

The binding of Pseudomonas aeruginosa to glycosphingolipids and to buccal and bronchial epithelial cells was analyzed. Three independently expressed specificities were found by bacterial binding to glycosphingolipids separated by thin-layer chromatography. All strains bound gangliotria- and gangliotetrasylceramide. All but one of the strains bound sialic acid-containing glycosphingolipids and lactosylceramide. The latter two specificities could be separated in that the lactosylceramide binding was retained and the sialic acid binding was suppressed when bovine serum albumin was used as a blocking agent in the thin-layer chromatography assay. The attachment to buccal epithelial cells, like the binding to sialylated compounds and lactosylceramide, was abolished by Formalin treatment of the bacteria, suggesting the importance of these specificities for cell adherence. In contrast, the binding to gangliotria- and gangliotetraosylceramide was retained by nonattaching Formalin-treated bacteria.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
Antigens
CD
Bacterial Adhesion
physiology
Carbohydrate Sequence
Epithelium
microbiology
Glycosphingolipids
metabolism
Humans
Lactosylceramides
Molecular Sequence Data
Mouth
microbiology
N-Acetylneuraminic Acid
Pseudomonas aeruginosa
metabolism
Sialic Acids
metabolism

Hansson, Gunnar C.,1951Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine(Swepub:gu)xhagun (author)
Leffler, Hakon (author)
Riise, Gerdt C.,1956 (author)
Svanborg-Edén, Catharina (author)
Göteborgs universitetMedicinska institutionen (creator_code:org_t)

By the author/editor: Baker, N; Hansson, Gunnar ...; Leffler, Hakon; Riise, Gerdt C., ...; Svanborg-Edén, C ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Infectious Medic ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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