Sökning: WFRF:(Magnusson Yvonne 1957)
> (2000-2004) >
Study of the beta1 ...
Study of the beta1 adrenergic receptor expression in human tissues: immunological approach.
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Hellgren, I (författare)
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- Sylvén, C (författare)
- Karolinska Institutet
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- Magnusson, Yvonne, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Hjärt-kärlinstitutionen,Cardiovascular Institute
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(creator_code:org_t)
- Pharmaceutical Society of Japan, 2000
- 2000
- Engelska.
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Ingår i: Biological & pharmaceutical bulletin. - : Pharmaceutical Society of Japan. - 0918-6158 .- 1347-5215. ; 23:6, s. 700-3
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Abstract
Ämnesord
Stäng
- Questions exist regarding tissue distribution of the beta1 adrenergic receptor (beta1-AR). The aim of this study was to investigate relative distribution patterns of the beta1-AR at the protein level in a variety of human tissues by Western blot analysis. The specificity of anti-peptide antibodies was confirmed both by Western blot with recombinant beta1-AR expressed as a membrane protein in E. coli and by immunoprecipitation of membranes from Sf9 cells infected with baculovirus to express the human recombinant beta1-AR. beta1-AR was found in all tissues examined. The relative amount of protein varied significantly between the tissues, from highest in lung and testis to very low in liver. beta1-ARs were rather abundant in heart, kidney, placenta, spleen and thyroid. These results reveal unique distribution of beta1-AR protein that suggests its tissue specific role. Moreover, our data demonstrate a high sensitivity of immunological detection that allows direct comparison of beta1-AR subtype expression and could be used for receptor study in biopsies available in limited amounts, such as human heart biopsy.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Amino Acid Sequence
- Animals
- Antibodies
- chemistry
- immunology
- Antibody Specificity
- Blotting
- Western
- Humans
- Molecular Sequence Data
- Organ Specificity
- Rabbits
- Receptors
- Adrenergic
- beta-1
- immunology
- metabolism
- Recombinant Proteins
- immunology
- metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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