Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice.

• The purpose of this study was to investigate the potential of intranasal (IN) immunization with Neisseria meningitides B proteoliposome (AFPL1) and AFPL1-derived cochleate (AFCo1), containing glycoprotein D (gD) of herpes simplex virus type 2 (HSV-2) for induction of protective immunity against genital herpes infection in mice. We could show that IN immunization with both AFPL1 and AFCo1 containing gD induced gD-specific IgG antibody and lymphoproliferative responses. However, IFN-gamma response could only be detected in CD4(+) splenic cells and genital lymph node cells of the AFCo1gD immunized mice upon recall antigen stimulation in vitro. Importantly, IN immunization with AFCo1gD could elicit a complete protection against an otherwise lethal vaginal challenge with HSV-2, while the AFPL1gD immunized mice were only partially protected. Further, we could show that the IFN-gamma response and protective immunity observed after IN immunization with AFCo1gD are mediated via the adaptor molecule myeloid differentiation factor 88. These data may have implications for the development of a mucosal vaccine against genital herpes.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Nyckelord

Administration

Intranasal

Animals

Antibodies

Viral

immunology

Antigens

Bacterial

immunology

pharmacology

CD4-Positive T-Lymphocytes

immunology

Female

Herpes Genitalis

genetics

immunology

prevention & control

Herpesvirus 2

Human

immunology

Immunization

Immunoglobulin G

immunology

Interferon-gamma

immunology

Liposomes

Mice

Mice

Knockout

Myeloid Differentiation Factor 88

genetics

immunology

Neisseria meningitidis

Serogroup B

immunology

Recombinant Proteins

genetics

immunology

pharmacology

Viral Envelope Proteins

genetics

immunology

pharmacology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)