Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1.

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Wada, Yoshinao (author)

Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1.

Article/chapterEnglish2010

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2010

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LIBRIS-ID:oai:gup.ub.gu.se/142042
https://gup.ub.gu.se/publication/142042URI
https://doi.org/10.1074/mcp.M900450-MCP200DOI

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Language:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

The Human Proteome Organisation Human Disease Glycomics/Proteome Initiative recently coordinated a multi-institutional study that evaluated methodologies that are widely used for defining the N-glycan content in glycoproteins. The study convincingly endorsed mass spectrometry as the technique of choice for glycomic profiling in the discovery phase of diagnostic research. The present study reports the extension of the Human Disease Glycomics/Proteome Initiative's activities to an assessment of the methodologies currently used for O-glycan analysis. Three samples of IgA1 isolated from the serum of patients with multiple myeloma were distributed to 15 laboratories worldwide for O-glycomics analysis. A variety of mass spectrometric and chromatographic procedures representative of current methodologies were used. Similar to the previous N-glycan study, the results convincingly confirmed the pre-eminent performance of MS for O-glycan profiling. Two general strategies were found to give the most reliable data, namely direct MS analysis of mixtures of permethylated reduced glycans in the positive ion mode and analysis of native reduced glycans in the negative ion mode using LC-MS approaches. In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
Algorithms
Carbohydrate Sequence
Disease
etiology
Glycomics
methods
organization & administration
standards
Glycoproteins
chemistry
Glycosylation
Humans
Immunoglobulin A
analysis
chemistry
metabolism
Metabolome
Models
Biological
Polysaccharides
chemistry
Proteome
analysis
metabolism
Proteomics
methods
organization & administration
standards
Societies
Scientific
organization & administration

Added entries (persons, corporate bodies, meetings, titles ...)

Dell, Anne (author)
Haslam, Stuart M (author)
Tissot, Bérangère (author)
Canis, Kévin (author)
Azadi, Parastoo (author)
Bäckström, Malin,1967Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xbamal (author)
Costello, Catherine E (author)
Hansson, Gunnar C.,1951Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xhagun (author)
Hiki, Yoshiyuki (author)
Ishihara, Mayumi (author)
Ito, Hiromi (author)
Kakehi, Kazuaki (author)
Karlsson, Niclas G.,1966 (author)
Hayes, C E (author)
Kato, Koichi (author)
Kawasaki, Nana (author)
Khoo, Kay-Hooi (author)
Kobayashi, Kunihiko (author)
Kolarich, Daniel (author)
Kondo, Akihiro (author)
Lebrilla, Carlito (author)
Nakano, Miyako (author)
Narimatsu, Hisashi (author)
Novak, Jan (author)
Novotny, Milos V (author)
Ohno, Erina (author)
Packer, Nicolle H (author)
Palaima, Elizabeth (author)
Renfrow, Matthew B (author)
Tajiri, Michiko (author)
Thomsson, Kristina A,1969Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xthokr (author)
Yagi, Hirokazu (author)
Yu, Shin-Yi (author)
Taniguchi, Naoyuki (author)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi (creator_code:org_t)

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In:Molecular & cellular proteomics9:4, s. 719-7271535-9484

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

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