Angiotensin II receptor expression following intestinal transplantation in mice.

• BACKGROUND: To further improve the success rate of intestinal transplantation there is a need to find early appearing indicators of rejection. The specific aim of this study was to compare Angiotensin (Ang) II type 1 receptor and Ang II type 2 receptor expression in relation to histological signs of rejection. METHODS: Mice of the C57BL6 strain with syngeneic intestinal grafts were compared to mice subjected to allogeneic intestinal transplantation with BalbC strain as donors. Local expression of Ang II type 1 and 2 receptor was evaluated using rt-PCR and Western blot and compared to histological picture in grafts and native intestine. RESULTS: The Ang II type 2 receptor protein expression was markedly up-regulated in the allogeneically transplanted graft from day 1 postoperatively. Histological signs of rejection were not seen until day 6. CONCLUSION: Intestinal allograft transplantation in mice is associated with a marked up-regulation of the Ang II type 2 receptor. However, the detailed role of the renin-angiotensin system in the immune rejection following intestinal transplantation remains to be clarified.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Anestesi och intensivvård (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Anesthesiology and Intensive Care (hsv//eng)

Nyckelord

Animals

Blotting

Western

Gene Expression

Graft Rejection

pathology

physiopathology

Graft Survival

physiology

Jejunum

pathology

physiology

transplantation

Male

Mice

Mice

Inbred BALB C

Mice

Inbred C57BL

Receptor

Angiotensin

Type 1

genetics

metabolism

Receptor

Angiotensin

Type 2

genetics

metabolism

Renin-Angiotensin System

physiology

Reverse Transcriptase Polymerase Chain Reaction

Up-Regulation

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)