Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: the importance of codon optimization and clone selection.

Öberg, Fredrik,1982Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry (författare)

In the last 15 years, 80% of all recombinant proteins reported in the literature were produced in the bacterium, Escherichia coli, or the yeast, Pichia pastoris. Nonetheless, developing effective general strategies for producing recombinant eukaryotic membrane proteins in these organisms remains a particular challenge. Using a validated screening procedure together with accurate yield quantitation, we therefore wished to establish the critical steps contributing to high yields of recombinant eukaryotic membrane protein in P. pastoris. Whilst the use of fusion partners to generate chimeric constructs and directed mutagenesis have previously been shown to be effective in bacterial hosts, we conclude that this approach is not transferable to yeast. Rather, codon optimization and the preparation and selection of high-yielding P. pastoris clones are effective strategies for maximizing yields of human aquaporins.

Sjöhamn, Jennie,1986Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry(Swepub:gu)xsjohj (författare)
Conner, Matthew T (författare)
Bill, Roslyn M.Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology (författare)
Hedfalk, Kristina,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry(Swepub:gu)xhedkr (författare)
Göteborgs universitetInstitutionen för kemi (creator_code:org_t)

Ingår i:Molecular membrane biology: Informa UK Limited28:6, s. 398-4111464-52030968-7688

Av författaren/redakt...: Öberg, Fredrik, ...; Sjöhamn, Jennie, ...; Conner, Matthew ...; Bill, Roslyn M.; Hedfalk, Kristin ...

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