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Revealing the genet...
Revealing the genetic structure of a trait by sequencing a population under selection.
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Parts, Leopold (author)
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Cubillos, Francisco A (author)
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- Warringer, Jonas, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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Jain, Kanika (author)
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Salinas, Francisco (author)
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Bumpstead, Suzannah J (author)
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- Molin, Mikael, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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Zia, Amin (author)
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Simpson, Jared T (author)
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Quail, Michael A (author)
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Moses, Alan (author)
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Louis, Edward J (author)
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Durbin, Richard (author)
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Liti, Gianni (author)
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(creator_code:org_t)
- 2011-03-21
- 2011
- English.
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In: Genome research. - : Cold Spring Harbor Laboratory. - 1549-5469 .- 1088-9051. ; 21:7, s. 1131-8
- Related links:
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http://genome.cshlp....
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- One approach to understanding the genetic basis of traits is to study their pattern of inheritance among offspring of phenotypically different parents. Previously, such analysis has been limited by low mapping resolution, high labor costs, and large sample size requirements for detecting modest effects. Here, we present a novel approach to map trait loci using artificial selection. First, we generated populations of 10-100 million haploid and diploid segregants by crossing two budding yeast strains of different heat tolerance for up to 12 generations. We then subjected these large segregant pools to heat stress for up to 12 d, enriching for beneficial alleles. Finally, we sequenced total DNA from the pools before and during selection to measure the changes in parental allele frequency. We mapped 21 intervals with significant changes in genetic background in response to selection, which is several times more than found with traditional linkage methods. Nine of these regions contained two or fewer genes, yielding much higher resolution than previous genomic linkage studies. Multiple members of the RAS/cAMP signaling pathway were implicated, along with genes previously not annotated with heat stress response function. Surprisingly, at most selected loci, allele frequencies stopped changing before the end of the selection experiment, but alleles did not become fixed. Furthermore, we were able to detect the same set of trait loci in a population of diploid individuals with similar power and resolution, and observed primarily additive effects, similar to what is seen for complex trait genetics in other diploid organisms such as humans.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- Alleles
- Chromosome Mapping
- DNA
- Fungal
- genetics
- Diploidy
- Gene Expression Regulation
- Gene Frequency
- Gene Library
- Genetic Linkage
- Genetics
- Population
- methods
- Genome
- Haploidy
- Haplotypes
- Models
- Biological
- Phenotype
- Quantitative Trait Loci
- Saccharomyces cerevisiae
- genetics
- growth & development
- Selection
- Genetic
- Sequence Analysis
- DNA
- methods
- Signal Transduction
- Temperature
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Parts, Leopold
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Cubillos, Franci ...
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Warringer, Jonas ...
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Jain, Kanika
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Salinas, Francis ...
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Bumpstead, Suzan ...
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show more...
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Molin, Mikael, 1 ...
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Zia, Amin
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Simpson, Jared T
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Quail, Michael A
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Moses, Alan
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Louis, Edward J
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Durbin, Richard
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Liti, Gianni
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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Genome research
- By the university
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University of Gothenburg