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Characterization of...
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Ekström, Karin,1978Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap,Krefting Research Centre,Institute of Clinical Sciences, Department of Biomaterials
(författare)
Characterization of mRNA and microRNA in human mast cell-derived exosomes and their transfer to other mast cells and blood CD34 progenitor cells
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/158635
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https://gup.ub.gu.se/publication/158635URI
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https://doi.org/10.3402/jev.v1i0.18389DOI
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Background: Exosomes are nanosized vesicles of endocytic origin that are released into the extracellular environment by many different cells. It has been shown that exosomes from various cellular origins contain a substantial amount of RNA (mainly mRNA and microRNA). More importantly, exosomes are capable of delivering their RNA content to target cells, which is a novel way of cell-to-cell communication. The aim of 20 this study was to evaluate whether exosomal shuttle RNA could play a role in the communication between human mast cells and between human mast cells and human CD34+progenitor cells. Methods: The mRNA and microRNA content of exosomes from a human mast cell line, HMC-1, was analysed by using microarray technology. Co-culture experiments followed by flow cytometry analysis and confocal microscopy as well as radioactive labeling experiments were performed to examine the uptake of 25 these exosomes and the shuttle of the RNA to other mast cells and CD34+ progenitor cells. Results: In this study, we show that human mast cells release RNA-containing exosomes, with the capacity to shuttle RNA between cells. Interestingly, by using microRNA microarray analysis, 116 microRNAs could be identified in the exosomes and 134 microRNAs in the donor mast cells. Furthermore, DNA microarray experiments revealed the presence of approximately 1800 mRNAs in the exosomes, which represent 15% of 30 the donor cell mRNA content. In addition, transfer experiments revealed that exosomes can shuttle RNA between human mast cells and to CD34+ hematopoietic progenitor cells. Conclusion: These findings suggest that exosomal shuttle RNA (esRNA) can play a role in the communication between cells, including mast cells and CD34+ progenitor cells, implying a role in cells maturation process. 35
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Valadi, Hadi,1963Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xvalha
(författare)
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Sjöstrand, Margareta,1947Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xsjosm
(författare)
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Malmhäll, Carina,1959Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xmalmc
(författare)
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Bossios, Apostolos,1969Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xbosap
(författare)
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Eldh, Maria,1980Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xeldhm
(författare)
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Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xlotja
(författare)
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Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Extracellular Vesicles (JEV): Wiley12001-3078
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Ekström, Karin, ...
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Bossios, Apostol ...
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Eldh, Maria, 198 ...
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Lötvall, Jan, 19 ...
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Göteborgs universitet