Endocytosis and trafficking of human lactoferrin in macrophage-like human THP-1 cells (1).

Different cell types have been reported to internalize lactoferrin (Lf) by specific or nonspecific receptors. Our studies focused on the endocytic pathway of human Lf in macrophage-like THP-1 cells. Lactoferrin was found to be internalized by THP-1 cells differentiated with phorbol myristate acetate. Incubation of cells with chlorpromazine and dansylcadaverine, inhibitors of clathrin-dependent endocytosis, led to a 50% inhibition of Lf internalization compared with untreated cells. Bafilomycin A1 and NH(4)Cl treatment also resulted in 40%-60% inhibition, respectively, suggesting that the internalization of Lf may partly be mediated by acidic endosome-like organelles. Endocytic uptake of Lf was also cholesterol-dependent, as shown by methyl-β-cyclodextrin or nystatin treatment of the cells prior to internalization. Partial colocalization of Lf and EEA-1, a marker specific for early endosomes, could be observed. Colocalization of Lf with a specific endoplasmic reticulum marker was also detected. Our results suggest that Lf is internalized mainly by the clathrin-dependent pathway in THP-1 cells and targets the ER. The physiological consequences of this intracellular trafficking will be the subject of future investigations.

Macovei, Alina (författare)
Sima, Livia (författare)
Nichita, Norica (författare)
Mattsby-Baltzer, Inger,1949Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xmatin (författare)
Roseanu, Anca (författare)
Göteborgs universitetInstitutionen för biomedicin (creator_code:org_t)

Ingår i:Biochemistry and cell biology = Biochimie et biologie cellulaire: Canadian Science Publishing90:3, s. 449-551208-6002
Ingår i:Biochemistry and Cell Biology: Canadian Science Publishing90:3, s. 449-550829-8211

Av författaren/redakt...: Florian, Paula; Macovei, Alina; Sima, Livia; Nichita, Norica; Mattsby-Baltzer, ...; Roseanu, Anca

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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