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Endocytosis and tra...
Endocytosis and trafficking of human lactoferrin in macrophage-like human THP-1 cells (1).
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Florian, Paula (author)
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Macovei, Alina (author)
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Sima, Livia (author)
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Nichita, Norica (author)
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- Mattsby-Baltzer, Inger, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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Roseanu, Anca (author)
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(creator_code:org_t)
- Canadian Science Publishing, 2012
- 2012
- English.
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In: Biochemistry and cell biology = Biochimie et biologie cellulaire. - : Canadian Science Publishing. - 1208-6002. ; 90:3, s. 449-55
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Different cell types have been reported to internalize lactoferrin (Lf) by specific or nonspecific receptors. Our studies focused on the endocytic pathway of human Lf in macrophage-like THP-1 cells. Lactoferrin was found to be internalized by THP-1 cells differentiated with phorbol myristate acetate. Incubation of cells with chlorpromazine and dansylcadaverine, inhibitors of clathrin-dependent endocytosis, led to a 50% inhibition of Lf internalization compared with untreated cells. Bafilomycin A1 and NH(4)Cl treatment also resulted in 40%-60% inhibition, respectively, suggesting that the internalization of Lf may partly be mediated by acidic endosome-like organelles. Endocytic uptake of Lf was also cholesterol-dependent, as shown by methyl-β-cyclodextrin or nystatin treatment of the cells prior to internalization. Partial colocalization of Lf and EEA-1, a marker specific for early endosomes, could be observed. Colocalization of Lf with a specific endoplasmic reticulum marker was also detected. Our results suggest that Lf is internalized mainly by the clathrin-dependent pathway in THP-1 cells and targets the ER. The physiological consequences of this intracellular trafficking will be the subject of future investigations.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- lactoferrin
- endocytosis
- clathrin-dependent pathway
- THP-1 cells
- ER
Publication and Content Type
- ref (subject category)
- art (subject category)
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