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Endocytosis and tra...
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Florian, Paula
(författare)
Endocytosis and trafficking of human lactoferrin in macrophage-like human THP-1 cells (1).
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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Canadian Science Publishing,2012
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/159306
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https://gup.ub.gu.se/publication/159306URI
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https://doi.org/10.1139/o11-090DOI
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Different cell types have been reported to internalize lactoferrin (Lf) by specific or nonspecific receptors. Our studies focused on the endocytic pathway of human Lf in macrophage-like THP-1 cells. Lactoferrin was found to be internalized by THP-1 cells differentiated with phorbol myristate acetate. Incubation of cells with chlorpromazine and dansylcadaverine, inhibitors of clathrin-dependent endocytosis, led to a 50% inhibition of Lf internalization compared with untreated cells. Bafilomycin A1 and NH(4)Cl treatment also resulted in 40%-60% inhibition, respectively, suggesting that the internalization of Lf may partly be mediated by acidic endosome-like organelles. Endocytic uptake of Lf was also cholesterol-dependent, as shown by methyl-β-cyclodextrin or nystatin treatment of the cells prior to internalization. Partial colocalization of Lf and EEA-1, a marker specific for early endosomes, could be observed. Colocalization of Lf with a specific endoplasmic reticulum marker was also detected. Our results suggest that Lf is internalized mainly by the clathrin-dependent pathway in THP-1 cells and targets the ER. The physiological consequences of this intracellular trafficking will be the subject of future investigations.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Macovei, Alina
(författare)
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Sima, Livia
(författare)
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Nichita, Norica
(författare)
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Mattsby-Baltzer, Inger,1949Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xmatin
(författare)
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Roseanu, Anca
(författare)
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Göteborgs universitetInstitutionen för biomedicin
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biochemistry and cell biology = Biochimie et biologie cellulaire: Canadian Science Publishing90:3, s. 449-551208-6002
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Ingår i:Biochemistry and Cell Biology: Canadian Science Publishing90:3, s. 449-550829-8211
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