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High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

Boutet, S. (author)
Lomb, L. (author)
Williams, G. J. (author)
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Barends, T. R. M. (author)
Aquila, A. (author)
Doak, R. B. (author)
Weierstall, U. (author)
Deponte, D. P. (author)
Steinbrener, J. (author)
Shoeman, R. L. (author)
Seibert, M Marvin (author)
Barty, A. (author)
White, T. A. (author)
Kassemeyer, S. (author)
Kirian, R. A. (author)
Seibert, M. M. (author)
Montanez, P. A. (author)
Kenney, C. (author)
Herbst, R. (author)
Hart, P. (author)
Pines, J. (author)
Haller, G. (author)
Gruner, S. M. (author)
Philipp, H. T. (author)
Tate, M. W. (author)
Hromalik, M. (author)
Koerner, L. J. (author)
van Bakel, N. (author)
Morse, J. (author)
Ghonsalves, W. (author)
Arnlund, David (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Bogan, M. J. (author)
Caleman, Carl (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Fromme, R. (author)
Hampton, C. Y. (author)
Hunter, M. S. (author)
Johansson, Linda C, 1983 (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Katona, Gergely, 1975 (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Kupitz, C. (author)
Liang, M. N. (author)
Martin, A. V. (author)
Nass, K. (author)
Redecke, L. (author)
Stellato, F. (author)
Timneanu, Nicusor (author)
Uppsala universitet,Molekylär biofysik
Wang, D. J. (author)
Zatsepin, N. A. (author)
Schafer, D. (author)
Defever, J. (author)
Neutze, Richard, 1969 (author)
Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
Fromme, P. (author)
Spence, J. C. H. (author)
Chapman, H. N. (author)
Schlichting, I. (author)
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 (creator_code:org_t)
American Association for the Advancement of Science (AAAS), 2012
2012
English.
In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 337:6092, s. 362-364
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Nanoteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Nano-technology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)

Keyword

diffraction data
nanocrystallography
lysozyme
crystals

Publication and Content Type

ref (subject category)
art (subject category)

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