Peptidome Analysis of Cerebrospinal Fluid by LC-MALDI MS

• We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000-4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

sclerosis-related proteins

alzheimers-disease

mass-spectrometry

multiple-sclerosis

serum peptidome

frontotemporal dementia

proteomic

analysis

identification

biomarkers

weight

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)