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Identification of a...
Identification of an intracellular M17 family leucine aminopeptidase that is required for virulence in Staphylococcus aureus
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Carroll, R. K. (author)
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Robison, T. M. (author)
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Rivera, F. E. (author)
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Davenport, J. E. (author)
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- Jonsson, Ing-Marie, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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Florczyk, D. (author)
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- Tarkowski, Andrej, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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Potempa, J. (author)
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Koziel, J. (author)
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Shaw, L. N. (author)
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
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In: Microbes and Infection. - : Elsevier BV. - 1286-4579. ; 14:11, s. 989-999
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https://europepmc.or...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Staphylococcus aureus is a highly virulent bacterial pathogen capable of causing a variety of ailments throughout the human body. It is a major public health concern due to the continued emergence of highly pathogenic methicillin resistant strains (MRSA) both within hospitals and in the community. Virulence in S. aureus is mediated by an array of secreted and cell wall associated virulence factors, including toxins, hemolysins and proteases. In this work we identify a (l) under bar eucine (a) under bar mino (p) under bar eptidase (LAP, pepZ) that strongly impacts the pathogenic abilities of S. aureus. Disruption of the pepZ gene in either Newman or USA300 resulted in a dramatic attenuation of virulence in both localized and systemic models of infection. LAP is required for survival inside human macrophages and gene expression analysis shows that pepZ expression is highest in the intracellular environment. We examine the cellular location of LAP and demonstrate that it is localized to the bacterial cytosol. These results identify for the first time an intracellular leucine aminopeptidase that influences disease causation in a Gram-positive bacterium. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Leucine aminopeptidase
- LAP
- Virulence
- Peptidase
- Staphylococcus
- site-specific recombination
- lactic-acid bacteria
- escherichia-coli
- systemic infection
- bacillus-subtilis
- peptidase-n
- gene
- pepa
- inactivation
- macrophages
- arlier d
- 1995
- journal of molecular biology
- v250
- p392
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Carroll, R. K.
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Robison, T. M.
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Rivera, F. E.
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Davenport, J. E.
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Jonsson, Ing-Mar ...
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Florczyk, D.
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show more...
-
Tarkowski, Andre ...
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Potempa, J.
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Koziel, J.
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Shaw, L. N.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Microbes and Inf ...
- By the university
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University of Gothenburg