Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli

• Establishment of a simple and rapid multiplex PCR system for identification of the main diarrheagenic E. coli categories, including enteroaggregative E. coil. enterotoxigenic E. coil, enteropathogenic E. coil, and enterohemorrhagic E. coil, is described. This two-step multiplex PCR system allows the identification by targeting CVD432, LT, STh, Sip, Eae, Bfp, Stx1, and Stx2. By applying the developed multiplex PCR system, categorization of E. coil isolates isolated from stool samples of infants with diarrhea into the main diarrheagenic E. coil categories is also shown. (C) 2011 Elsevier GmbH. All rights reserved.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

Nyckelord

Diarrheagenic E. coli

Multiplex PCR

young-children

prevalence

diagnosis

shigella

vietnam

strains

infants

hanoi

assay

epec

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)