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- Rekabdar, Elham,1971Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology (author)
Variability of the glycoprotein G gene in clinical isolates of herpes simplex virus type 1.
- Article/chapterEnglish1999
Publisher, publication year, extent ...
- 1999
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/179829
- https://gup.ub.gu.se/publication/179829URI
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- Language:English
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- Glycoprotein G (gG) of herpes simplex virus type 1 (HSV-1) has been used as a prototype antigen for HSV-1 type-specific serodiagnosis, but data on the sequence variability of the gene coding for this protein in wild-type strains are lacking. In this study, direct DNA sequencing of the gG-1 genes from PCR products was performed with clinical HSV-1 isolates from 11 subjects as well as with strains Syn 17(+), F, and KOS 321. The reference strains Syn 17(+) and F showed a high degree of conservation, while KOS 321 carried 13 missense mutations and, in addition, 12 silent mutations. Three clinical isolates showed mutations leading to amino acid alterations: one had a mutation of K(122) to N, which is a gG-1-to-gG-2 alteration; another contained all mutations which were observed in KOS 321 except two silent mutations; and the third isolate carried five missense mutations. Two clinical isolates as well as strain KOS 321 showed a mutation (F(111)-->V) within the epitope of a gG-1-reactive monoclonal antibody (MAb). When all viruses were tested for reactivity with the anti-gG-1 MAb, the three strains with the F(111)-->V mutation were found to be unreactive. Furthermore, gG-1 antibodies purified from sera from the two patients carrying strains mutated in this epitope were less reactive when they were tested by an HSV-1-infected-cell assay. Therefore, our finding that the sequence variability of the gG-1 gene also affects B-cell epitope regions of this protein in clinical isolates may have consequences for the use of this protein as a type-specific antigen for serodiagnosis.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
- Adult
- Aged
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Antibodies
- Monoclonal
- Antibodies
- Viral
- immunology
- Antigens
- Surface
- analysis
- immunology
- Cells
- Cultured
- Cercopithecus aethiops
- DNA Mutational Analysis
- DNA
- Viral
- analysis
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- immunology
- Female
- Genetic Variation
- Herpes Simplex
- genetics
- immunology
- Herpesvirus 1
- Human
- genetics
- immunology
- Humans
- Immunoglobulin G
- immunology
- Kidney
- cytology
- Male
- Middle Aged
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Serologic Tests
- Viral Envelope Proteins
- genetics
- immunology
Added entries (persons, corporate bodies, meetings, titles ...)
- Tunbäck, Petra,1965Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för dermatologi och venereologi,Institute of Selected Clinical Sciences, Department of Dermatology and Venereology(Swepub:gu)xtunpe (author)
- Liljeqvist, Jan-Åke,1954Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology(Swepub:gu)xljanl (author)
- Bergström, Tomas,1950Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology(Swepub:gu)xberto (author)
- Göteborgs universitetInstitutionen för laboratoriemedicin, Avdelningen för klinisk virologi (creator_code:org_t)
Related titles
- In:Clinical and diagnostic laboratory immunology6:6, s. 826-311071-412X
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