Peptide structural analysis using continuous Ar cluster and C60 ion beams

A novel application of time-of-flight secondary ion mass spectrometry (ToF-SIMS) with continuous Ar cluster beams to peptide analysis was investigated. In order to evaluate peptide structures, it is necessary to detect fragment ions related to multiple neighbouring amino acid residues. It is, however, difficult to detect these using conventional ToF-SIMS primary ion beams such as Bi cluster beams. Recently, C60 and Ar cluster ion beams have been introduced to ToF-SIMS as primary ion beams and are expected to generate larger secondary ions than conventional ones. In this study, two sets of model peptides have been studied: (des-Tyr)-Leuenkephalin and (des-Tyr)-Met-enkephalin (molecular weights are approximately 400 Da), and [Asn1 Val5]-angiotensin II and [Val5]-angiotensin I (molecular weights are approximately 1,000 Da) in order to evaluate the usefulness of the large cluster ion beams for peptide structural analysis. As a result, by using the Ar cluster beams, peptide molecular ions and large fragment ions, which are not easily detected using conventional ToF-SIMS primary ion beams such as Bi3+, are clearly detected. Since the large fragment ions indicating amino acid sequences of the peptides are detected by the large cluster beams, it is suggested that the Ar cluster and C60 ion beams are useful for peptide structural analysis.

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
Continuous Ar cluster beam . ToF-SIMS . Peptide structure . (des-Tyr)-enkephalin . Angiotensin
Continuous Ar cluster beam . ToF-SIMS . Peptide structure . (des-Tyr)-enkephalin . Angiotensin

Fletcher, John S.Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)fletcher (author)
Sheraz, Sadia (author)
Kawashima, Tomoko (author)
Berrueta Razo, Irma (author)
Henderson, Alex (author)
Lockyer, Nicholas P (author)
Vickerman, John C (author)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

In:Analytical and Bioanalytical Chemistry: Springer Science and Business Media LLC405:21, s. 6621-66281618-26421618-2650

By the author/editor: Aoyagi, Satoka; Fletcher, John S ...; Sheraz, Sadia; Kawashima, Tomok ...; Berrueta Razo, I ...; Henderson, Alex; show more...; Lockyer, Nichola ...; Vickerman, John ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

By the university: University of Gothenburg; Chalmers University of Technology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.