Cross-species amplification and development of microsatellites for six species of European Coenagrionid damselflies

We describe the cross-amplification and development of new loci for six species of closely related European damselflies. First, twenty-nine published microsatellites for the damselflies Coenagrion puella and C. mercuriale were multiplexed using M13-tagged primers, tested on 23 individuals, and then cross-species amplified on 21-26 individuals of C. armatum, C. johanssoni, C. pulchellum and C. scitulum. Second, sixteen new primers were developed for use in C. armatum, C. johanssoni and C. scitulum, and screened on 21 individuals. Values for observed heterozygosities and number of alleles ranged between 0.00-0.87 and 2-19 respectively (over all loci and species). For all species the tested loci provide a minimum of 1-8 usable markers for population genetic studies.

Ingvarsson, Pär KUmeå universitet,Institutionen för ekologi, miljö och geovetenskap,Umeå Plant Science Centre (UPSC)(Swepub:umu)pain0001 (author)
Johansson, FrankUppsala universitet,Populationsbiologi och naturvårdsbiologi(Swepub:uu)frafr730 (author)
Umeå universitetInstitutionen för ekologi, miljö och geovetenskap (creator_code:org_t)

In:Conservation Genetics Resources: Springer Science and Business Media LLC4:1, s. 191-1961877-72521877-7260

By the author/editor: Johansson, Helen ...; Ingvarsson, Pär ...; Johansson, Frank

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