Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli

• Lysine acetylation is a frequently occurring post-translational modification in bacteria; however, little is known about its origin and regulation. Using the model bacterium Escherichia coil (E. coil), we found that most acetylation occurred at a low level and accumulated in growth-arrested cells in a manner that depended on the formation of acetyl-phosphate (AcP) through glycolysis. Mutant cells unable to produce AcP had significantly reduced acetylation levels, while mutant cells unable to convert AcP to acetate had significantly elevated acetylation levels. We showed that AcP can chemically acetylate lysine residues in vitro and that AcP levels are correlated with acetylation levels in vivo, suggesting that AcP may acetylate proteins nonenzymatically in cells. These results uncover a critical role for AcP in bacterial acetylation and indicate that most acetylation in E. coil occurs at a low level and is dynamically affected by metabolism and cell proliferation in a global, uniform manner.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Nyckelord

ESCHERICHIA-COLI

COA SYNTHETASE

ACETATE

IDENTIFICATION

EXPRESSION

PROTEOMICS

DIVERSITY

SUBSTRATE

USS BM

1994

MOLECULAR MICROBIOLOGY

V12

P973

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