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Isolectins from Sol...
Isolectins from Solanum tuberosum with different detailed carbohydrate binding specificities: unexpected recognition of lactosylceramide by N-acetyllactosamine-binding lectins.
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Ciopraga, J (author)
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- Ångström, Jonas, 1950 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
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- Bergström, Jörgen, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
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- Larsson, Thomas, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
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- Karlsson, Niclas G., 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
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Motas, C (author)
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Gozia, O (author)
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- Teneberg, Susann, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
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(creator_code:org_t)
- 2000
- 2000
- English.
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In: Journal of biochemistry. - 0021-924X. ; 128:5, s. 855-67
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Glycosphingolipid recognition by two isolectins from Solanum tuberosum was compared by the chromatogram binding assay. One lectin (PL-I) was isolated from potato tubers by affinity chromatography, and identified by MALDI-TOF mass spectrometry as a homodimer with a subunit molecular mass of 63,000. The other (PL-II) was a commercial lectin, characterized as two homodimeric isolectins with subunit molecular masses of 52,000 and 55,000, respectively. Both lectins recognized N-acetyllactosamine-containing glycosphingolipids, but the fine details of their carbohydrate binding specificities differed. PL-II preferentially bound to glycosphingolipids with N-acetyllactosamine branches, as Galbeta4GlcNAcbeta6(Galbeta4GlcNAcbeta3)Galbeta4Glcbeta1C er. PL-I also recognized this glycosphingolipid, but bound equally well to the linear glycosphingolipid Galbeta4GlcNAcbeta3Galbeta4GlcNAcbeta3Galbeta4Glcbeta1Cer. Neolactotetraosylceramide and the B5 pentaglycosylceramide were also bound by PL-I, while other glycosphingolipids with only one N-acetyllactosamine unit were non-binding. Surprisingly, both lectins also bound to lactosylceramide, with an absolute requirement for sphingosine and non-hydroxy fatty acids. The inhibition of binding to both lactosylceramide and N-acetyllactosamine-containing glycosphingolipids by N-acetylchitotetraose suggests that lactosylceramide is also accomodated within the N-acetylchitotetraose/N-acetyllactosamine-binding sites of the lectins. Through docking of glycosphingolipids onto a three-dimensional model of the PL-I hevein binding domain, a Galbeta4GlcNAcbeta3Galbeta4 binding epitope was defined. Furthermore, direct involvement of the ceramide in the binding of lactosylceramide was suggested.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Amino Sugars
- metabolism
- Antigens
- CD
- Antimicrobial Cationic Peptides
- Carbohydrate Metabolism
- Carbohydrate Sequence
- Electrophoresis
- Polyacrylamide Gel
- Lactosylceramides
- metabolism
- Lectins
- chemistry
- isolation & purification
- metabolism
- Magnetic Resonance Spectroscopy
- Models
- Molecular
- Molecular Sequence Data
- Plant Lectins
- Plant Proteins
- chemistry
- Sequence Alignment
- Solanum tuberosum
- chemistry
- Structure-Activity Relationship
Publication and Content Type
- ref (subject category)
- art (subject category)
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Ciopraga, J
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Ångström, Jonas, ...
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Bergström, Jörge ...
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Larsson, Thomas, ...
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Karlsson, Niclas ...
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Motas, C
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show more...
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Gozia, O
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Teneberg, Susann ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Other Basic Medi ...
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Journal of bioch ...
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University of Gothenburg